NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE3173 Query DataSets for GSE3173
Status Public on Aug 24, 2005
Title Identification of novel transcriptional networks in response to the treatment D3T (3H-1, 2-dithiole-3-thione)
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary 3H-1, 2-dithiole-3-thione (D3T), an inducer of antioxidant and phase 2 genes, is known to enhance the detoxification of environmental carcinogens, prevent neoplasia, and to elicit other protective effects. However, a comprehensive view of the regulatory pathways induced by this compound has not yet been elaborated. Fischer F344 rats were gavaged daily for 5 days with vehicle or D3T (0.3 mmol/kg). The global changes of gene expression in liver were measured with Affymetrix RG-U34A chips. Using functional class scoring, a semi-supervised method exploring both the expression pattern and the functional annotation of the genes, the Gene Ontology classes were ranked according to the significance of the impact of D3T treatment. Two unexpected functional classes were identified for the D3T treatment, cytosolic ribosome constituents with 90% of those genes increased, and cholesterol biosynthesis with 91% of the genes repressed. In another novel approach, the differentially expressed genes were evaluated by the Ingenuity computational pathway analysis tool to identify specific regulatory networks and canonical pathways responsive to D3T treatment. In addition to the known glutathione metabolism pathway (ρ = 0.0011), several other significant pathways were also revealed, including antigen presentation (ρ = 0.000476), androgen/estrogen biosynthesis (ρ = 0.000551), fatty acid (ρ = 0.000216) and tryptophan metabolism (ρ = 0.000331) pathways. These findings showed a profound impact of D3T on lipid metabolism and anti-inflammatory/immune-suppressive response, indicating broader cytoprotective effect of this compound than previously expected.
Keywords: Response to antioxidant compound
 
Overall design Total RNA were isolated from either vehicle or D3T (0.3mmol/kg body weight) treated rat liver, 4 rats for each group, hybridized on 8 Affymetrix RG-U34A chips
 
Contributor(s) Huang Y, Yan J, Lubet R, Kensler TW, Sutter TR
Citation(s) 16317079
Submission date Aug 22, 2005
Last update date Feb 21, 2017
Contact name Yong Huang
E-mail(s) yh9fj@virginia.edu
Phone (434) 243-0842
Organization name University of Viginia
Department Medicine
Street address 1340 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platforms (1)
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
Samples (8)
GSM71311 Liver_Control _Rep1
GSM71368 Liver_Control _Rep2
GSM71369 Liver_Control _Rep3
Relations
BioProject PRJNA92715

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap