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Series GSE3181 Query DataSets for GSE3181
Status Public on Dec 21, 2005
Title SRF-null neonatal cardiomyocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Serum Response Factor (SRF) is a transcriptional regulator required for mesodermal development. Numerous studies have implicated SRF as a central regulator of muscle gene expression and myogenesis. In this present study we used a loss of function approach to delineate the role of SRF in cardiac myocyte gene expression and function. In SRF null neonatal cardiomyocytes, we observe severe defects in the contractile apparatus, including Z-disc and stress fiber formation, as well as mislocalization and/or attenuation of sarcomeric proteins. Consistent with this, gene array and RT-PCR analyses show downregulation of genes encoding key cardiac transcriptional regulatory factors and proteins required for the maintenance of sarcomeric structure, function, and regulation. Chromatin IP analysis reveals that at least a subset of these proteins are likely regulated directly by SRF. Together the results presented here reveal new cellular and genetic mechanisms through which SRF exacts control over the contractile apparatus in cardiac myocytes.
Keywords: genetic modification
Overall design There are three control replicates (cardiomyocytes transduced with Adeno-GFP virus) and three experimental replicates (cardiomyocytes transduced with Adeno-CRE to excise SRF).
Contributor(s) Balza RO, Misra RP
Citation(s) 16368687
Submission date Aug 23, 2005
Last update date Feb 11, 2019
Contact name Ravi P. Misra
Fax 414 456-6510
Organization name Medical College of Wisconsin
Department Biochemistry
Street address 8701 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM71592 cardiomyocyte control 1
GSM71606 Cardiomyocyte control 2
GSM71608 cardiomyocyte control 3
BioProject PRJNA92729

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