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Series GSE3196 Query DataSets for GSE3196
Status Public on Oct 24, 2005
Title Gene expression in hypoxic MEFs having only p300 and CBP with deleted CH1 domains
Organism Mus musculus
Experiment type Expression profiling by array
Summary The CH1 protein interaction domain of the transcriptional coactivators p300 and CBP is thought to interact with HIF-1alpha and this interaction is thought to be critical to the expression of HIF-1alpha target genes in response to hypoxia. To test the requirement of the CH1 domain for gene expression in response to hypoxia, rimary mouse embryonic fibroblasts (MEFs) were generated from C57Bl/6x129/Sv F2 e14.5 embryos that contain a deletion in the CH1 domain of three of four alleles of CBP and p300. The remaining allele of p300 or CBP was a conditional knock out allele. Control MEFs with only a single conditional knockout allele of p300 or CBP were also generated. At passage 3 MEFs were infected with Cre Adenovirus and grown until they had expanded at least 100 fold. Subconfluent MEFs were treated with 21% oxygen (normoxia) or 0.1% oxygen (hypoxia) with 5% carbon dioxide at 37 C in a humid chamber for 6hrs. At the start of treatment, medium was removed and replaced with medium (DMEM+10% FBS+pen-strep+ l-glu) that had been preequilibrated overnight in normoxia or hypoxia as appropriate. Immediately after treatment, cells were lysed in Trizol for RNA extraction.
Keywords: genetic modification, dose response
Overall design 12 samples; 4 genotypes [CBP+/flox (flox1), p300 +/flox (flox2), CBP CH1/flox;p300 CH1/CH1 (triCH1flox1),CBP CH1/CH1;p300 CH1/flox (triCH1flox2)] , 2 treatments (normoxia and hypoxia).
Contributor(s) Kasper LH, Boussouar F, Boyd K, Xu W, Biesen MA, Rehg J, Baudino TA, Cleveland JL, Brindle PK
Citation(s) 16237459
Submission date Aug 24, 2005
Last update date Feb 11, 2019
Contact name Paul K. Brindle
Phone 901 495 2451
Organization name St Jude Children's Research Hospital
Department Biochemistry
Street address 332 N Lauderdale
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM67326 flox2_1_normoxia
GSM67327 triCH1flox2_1_normoxia
GSM67328 flox1_normoxia
BioProject PRJNA92857

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