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Series GSE3203 Query DataSets for GSE3203
Status Public on Aug 26, 2006
Title Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN
Organism Mus musculus
Experiment type Expression profiling by array
Summary Influenza virus infection-induced gene expression changes of regional B cells are mediated at least in part through type I Interferon:
Our objective is to determine whether the influenza virus-infection induced gene expression changes in regional lymph node B cells are facilitated at least in part through type I interferon. Our specific aim is to compare the gene expression profile of highly FACS-purified B cells in the regional lymph nodes of wildtype and IFNR-/- mice prior to and 48h following infection with influenza virus infection and to contrast this expression profile with that of FACS-purified wildtype B cells activated in vitro with IFN-beta +/- anti-CD86 for 12h.
Keywords: Infection-induced responses
Overall design We analyzed gene expression from mouse lymph node B cells purified by flow cytometric sorting using single channel oligonucleotide microarrays. There were 4 groups: 1) wild type uninfected mice (control group), 2) wild type mice infected with influenza (flu) for 2 days, 3) IFNR-/- mice infected with flu for 2 days, 4) cells stimulated with IFN-b in vitro for 17 h. Each group contained 4 biological replicates obtained from independent experiments. There were 16 total samples and each was measured on a separate array.
Contributor(s) Coro ES, Chang WL, Doucett VP, Xiao Y, Erle DJ, Baumgarth N
Citation(s) 17237394
Submission date Aug 25, 2005
Last update date Feb 11, 2019
Contact name Michael E. Salazar
Phone (415) 514-4371
Organization name University of California, San Francisco
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM71992 FACS-purified B cells_wildtype_non-infected_rep1
GSM71993 FACS-purified B cells_wildtype_non-infected_rep2
GSM71994 FACS-purified B cells_129SV/EV_non-infected_rep3
BioProject PRJNA92871

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Supplementary file Size Download File type/resource
GSE3203_RAW.tar 99.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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