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Series GSE32034 Query DataSets for GSE32034
Status Public on Oct 03, 2012
Title Tissue-specific differences in PPARγ control of macrophage function.
Organism Mus musculus
Experiment type Expression profiling by array
Summary PPARγ is known for its anti-inflammatory actions in macrophages. However, which macrophage populations express PPARγ in vivo and how it regulates tissue homeostasis in the steady state and during inflammation is not completely understood. We show that lung and spleen macrophages constitutively expressed PPARγ, while other macrophage populations did not. Recruitment of monocytes to sites of inflammation was associated with induction of PPARγ as they differentiated to macrophages. Its absence in these macrophages led to failed resolution of inflammation, characterized by persistent, low-level recruitment of leukocytes. Conversely, PPARγ agonists supported an earlier cessation in leukocyte recruitment during resolution of acute inflammation and likewise suppressed monocyte recruitment to chronically inflamed atherosclerotic vessels. In the steady state, PPARγ deficiency in macrophages had no obvious impact in the spleen but profoundly altered cellular lipid homeostasis in lung macrophages. Reminiscent of pulmonary alveolar proteinosis, LysM-Cre x PPARγflox/flox mice displayed mild leukocytic inflammation in the steady-state lung and succumbed faster to mortality upon infection with S. pneumoniae. Surprisingly, this mortality was not due to overly exuberant inflammation, but instead to impaired bacterial clearance. Thus, in addition to its anti-inflammatory role in promoting resolution of inflammation, PPARγ sustains functionality in lung macrophages and thereby has a pivotal role in supporting pulmonary host defense.
 
Overall design The two major subsets of monocytes (Ly-6C+ and Ly-6Clo) from 12-week old C57Bl/6 mice were sorted and the RNA extracted and hybridized to Affymetrix GeneChip® 430 2.0 arrays. We pooled leukocytes from 5 mice for each sort and sorted 3 to 4 separate times for 3 to 4 biological replicates.
 
Contributor(s) Gautier EL, Chow A, Spanbroek R, Hutchison SB, Potteaux S, Greter M, Leboeuf M, Jakubzick C, Bogunovic M, van Rooijen N, Habenicht AJ, Merad M, Randolph GJ
Citation(s) 22855714
Submission date Sep 09, 2011
Last update date Feb 11, 2019
Contact name Emmanuel GAUTIER
E-mail(s) emmanuel.gautier@mssm.edu
Organization name Mount Sinai School of Medecine
Street address 1425 Madison ave
City New York
ZIP/Postal code 10029
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (14)
GSM794238 CTRL 1 week Ly6Chi monocyte #1
GSM794239 CTRL 1 week Ly6Chi monocyte #2
GSM794240 CTRL 1 week Ly6Chi monocyte #3
Relations
BioProject PRJNA147493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32034_RAW.tar 54.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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