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| Status |
Public on Oct 05, 2011 |
| Title |
Gene expression in Rspo2 and/or Wnt3a treated C57MG mouse mammary epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
R-spondins (Rspos) comprise a family of four secreted proteins that have important roles in cell proliferation, cell fate determination and organogenesis. Rspos typically exert their effects by potentiating the Wnt/β-catenin signaling pathway. To systematically investigate the impact of Rspo/Wnt on gene expression, we performed a microarray analysis using C57MG mouse mammary epithelial cells treated with recombinant Rspo2 and/or Wnt3a.
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| Overall design |
This study compares gene expression in C57MG cells after 24h treatment with recombinant Rspo2 and recombinant Wnt3a alone or in combination versus BSA treatment. Control cells were treated with BSA, since it was used as carrier for the recombinant proteins. Each treatment was done in biological triplicate.
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| Contributor(s) |
Baljinnyam B, Callahan R, Rubin JS |
| Citation(s) |
22238613 |
| Submission date |
Sep 13, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Jeffrey S Rubin |
| E-mail(s) |
rubinj@mail.nih.gov
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| Phone |
301-496-4265
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| Fax |
301-496-8479
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| Organization name |
NIH/NCI
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| Department |
CCR
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| Lab |
LCMB
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| Street address |
37 Convent Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (12)
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| GSM795626 |
C57MG cells treated with BSA, biological rep1 |
| GSM795627 |
C57MG cells treated with BSA, biological rep2 |
| GSM795628 |
C57MG cells treated with BSA, biological rep3 |
| GSM795629 |
C57MG cells treated with rRspo2, biological rep1 |
| GSM795630 |
C57MG cells treated with rRspo2, biological rep2 |
| GSM795631 |
C57MG cells treated with rRspo2, biological rep3 |
| GSM795632 |
C57MG cells treated with rWnt3a, biological rep1 |
| GSM795633 |
C57MG cells treated with rWnt3a, biological rep2 |
| GSM795634 |
C57MG cells treated with rWnt3a, biological rep3 |
| GSM795635 |
C57MG cells treated with rRspo2/rWnt3a, biological rep1 |
| GSM795636 |
C57MG cells treated with rRspo2/rWnt3a, biological rep2 |
| GSM795637 |
C57MG cells treated with rRspo2/rWnt3a, biological rep3 |
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| Relations |
| BioProject |
PRJNA147547 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32096_RAW.tar |
96.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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