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Series GSE3212 Query DataSets for GSE3212
Status Public on Sep 26, 2005
Title Gene expression in alveolar macrophages from phenotypically normal smokers compared to non-smokers
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cigarette smoking is the leading cause of the respiratory diseases collectively known as chronic obstructive pulmonary disease (COPD). While the pathogenesis of COPD is complex, there is abundant evidence that alveolar macrophages (AM) play an important role. Based on the concept that COPD is a slow-progressing disorder likely involving multiple mediators released by AM activated by cigarette smoke, the present study focuses on the identification of previously unrecognized genes that may be linked to early events in the molecular pathogenesis of COPD, as opposed to factors associated with the presence of disease. To accomplish this, microarray analysis using Affymetrix microarrays was used to carry out an unbiased survey of the differences in gene expression profiles in the AM of phenotypically normal, ~20 pack-yr smokers compared to healthy non-smokers. Although smoking did not alter the global gene expression pattern of AM, 75 genes were modulated by smoking, with 40 genes up-regulated and 35 down-regulated in the AM of smokers compared to non-smokers. Most of these genes belong to the functional categories of immune/inflammatory response, cell adhesion and extracellular matrix, proteolysis and antiproteolysis, lysosomal function, antioxidant-related, signal transduction and regulation of transcription. Of these 75 genes, 69 have not been previously recognized to be up- or down-regulated in alveolar macrophages in association with smoking or COPD, including genes coding for proteins belonging to all of the above categories, and others belonging to various functional categories or of unknown function. These observations suggest that gene expression responses of alveolar macrophages associated with the stress of cigarette smoking are more complex than previously thought, and offer a variety of new insights into the complex pathogenesis of smoking-induced lung diseases.
Keywords: Comparison of gene expression profile in smokers vs non-smokers
Overall design 5 non smokers and 5 smokers
Alveolar macrophages were obtained from bronchoalveolar lavage
Contributor(s) Heguy A, O' Connor T, Worgall S, Harvey B, Hackett N, Crystal R
Citation(s) 16520944
Submission date Aug 26, 2005
Last update date Jul 08, 2016
Contact name Yael Strulovici-Barel
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (1)
GPL80 [Hu6800] Affymetrix Human Full Length HuGeneFL Array
Samples (10)
GSM71942 NS1_NS010112
GSM71991 NS2_NS010119
GSM72243 NS3_NS010823
This SubSeries is part of SuperSeries:
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD
BioProject PRJNA104805

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3212_RAW.tar 19.8 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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