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Series GSE3212

Query DataSets for GSE3212

Status

Public on Sep 26, 2005

Title

Gene expression in alveolar macrophages from phenotypically normal smokers compared to non-smokers

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

Cigarette smoking is the leading cause of the respiratory diseases collectively known as chronic obstructive pulmonary disease (COPD). While the pathogenesis of COPD is complex, there is abundant evidence that alveolar macrophages (AM) play an important role. Based on the concept that COPD is a slow-progressing disorder likely involving multiple mediators released by AM activated by cigarette smoke, the present study focuses on the identification of previously unrecognized genes that may be linked to early events in the molecular pathogenesis of COPD, as opposed to factors associated with the presence of disease. To accomplish this, microarray analysis using Affymetrix microarrays was used to carry out an unbiased survey of the differences in gene expression profiles in the AM of phenotypically normal, ~20 pack-yr smokers compared to healthy non-smokers. Although smoking did not alter the global gene expression pattern of AM, 75 genes were modulated by smoking, with 40 genes up-regulated and 35 down-regulated in the AM of smokers compared to non-smokers. Most of these genes belong to the functional categories of immune/inflammatory response, cell adhesion and extracellular matrix, proteolysis and antiproteolysis, lysosomal function, antioxidant-related, signal transduction and regulation of transcription. Of these 75 genes, 69 have not been previously recognized to be up- or down-regulated in alveolar macrophages in association with smoking or COPD, including genes coding for proteins belonging to all of the above categories, and others belonging to various functional categories or of unknown function. These observations suggest that gene expression responses of alveolar macrophages associated with the stress of cigarette smoking are more complex than previously thought, and offer a variety of new insights into the complex pathogenesis of smoking-induced lung diseases.
Keywords: Comparison of gene expression profile in smokers vs non-smokers

Overall design

5 non smokers and 5 smokers
Alveolar macrophages were obtained from bronchoalveolar lavage

Contributor(s)

Heguy A, O' Connor T, Worgall S, Harvey B, Hackett N, Crystal R

Citation(s)

16520944

Submission date

Aug 26, 2005

Last update date

Jul 08, 2016

Contact name

Yael Strulovici-Barel