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| Status |
Public on May 22, 2012 |
| Title |
Retinal gene expression of low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Retinal gene expression was assessed from postnatal day (P) 8 old low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice with a Illumina mouse-WG6 expression BeadChip.
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| Overall design |
RNA was extracted from the retinas of P8 Lrp5 knockout and wild type mice (n=3 each group). Total RNA (1ug each) were reverse transcribed, followed by a single in vitro transcription amplification to incorporate biotin-labeled nucleotide, and subsequent hybridization and staining with strepatavidin-Cy3 according to the manufacturer’s instructions.
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| |
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| Contributor(s) |
Chen J, Smith LE |
| Citation(s) |
22272305 |
| Submission date |
Sep 15, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Jing Chen |
| E-mail(s) |
jing.chen@childrens.harvard.edu
|
| Organization name |
Children's Hospital Boston/Harvard Medical School
|
| Department |
Ophthalmology
|
| Street address |
300 Longwood Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA147657 |
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