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Series GSE32164 Query DataSets for GSE32164
Status Public on Sep 16, 2011
Title Expression data from macrophage maturation and polarization c-Myc in alternative macrophage activation experiments
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In response to microenvironmental signals macrophages undergo different activation, indicated as classic/M1 and alternative/M2 polarization. C-Myc transcription factor could be an essential player in M2 polarization.
Functional relevance of c-Myc in M2 macrophage biology is investigated by evaluating the effect of 100-58F4, on the transcriptional profile induced on human macrophages by IL-4.
 
Overall design Human monocytes were obtained from normal donor buffy coats by two-step gradient centrifugation using Ficoll (Biochrom) and Percoll (Amersham). Non-adherent cells were discarded, and the purified monocytes were incubated for 7 days in RPMI 1640 (Biochom) supplemented with 10% FCS (HyClone) and 100 ng/mL M-CSF to obtain resting macrophages. Macrophage polarization was obtained by removing the culture medium and culturing cells in RPMI 1640 supplemented with 10% FCS and 100 ng/mL LPS plus 20 ng/mL IFN-gamma (M1 polarization) or 20 ng/mL IL-4 (M2 polarization) for 24 h. When needed, chemical inhibitors were added with IL-4.
 
Contributor(s) Pello OM
Citation(s) 22067385
Submission date Sep 15, 2011
Last update date Jul 26, 2018
Contact name Massimo Locati
E-mail(s) transcriptional.profiles@humanitasresearch.it
Phone +39 02 8224 5116
Fax 02-82245101
URL http://www.humanitas.it/hur/cms/english/activities/lab/leukocyte_biology/groupleader.html
Organization name Istituto Clinico Humanitas
Department Department of Translational Medicine of the University of Milan Faculty of Medicine, IRCCS Istituto Clinico Humanitas
Lab Laboratory of Leukocyte Biology
Street address Via Manzoni, 113
City Rozzano
State/province MILAN
ZIP/Postal code 20089
Country Italy
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (9)
GSM797441 Resting fully differentiated macrophages (7 days of culture), rep 1
GSM797442 Alternative activated macrophages (M2) rep 1
GSM797443 Macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors, rep 1
Relations
BioProject PRJNA147641

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Supplementary file Size Download File type/resource
GSE32164_RAW.tar 34.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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