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| Status |
Public on Sep 14, 2012 |
| Title |
A549 expression profile induced by high levels of extracellular GPBP-1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Patients undergoing immune complex-mediated GN display increased levels of circulating GPBP-1 (cGPBP-1). To assess the relationship between high levels of cGPBP-1 and the onset of these diseases we incubated A549 cells with 200 ng/mL of recombinant extracellular GPBP-1 or with buffer (control). After 3 hours cells were lysed and total RNA extracted and assessed the mRNA expression. The functional analysis of data provide evidence that high levels of circulating GPBP-1 correlate with a lupus-prone cellular response.
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| Overall design |
A549 cells were seeded in 6-well plates, grown for 18 hours and treated with 200 ng/mL of recombinant extracellular GPBP-1 or buffer (TBS). After three hours of incubation, cells were lysed, total RNA extracted and mRNA expression analysed following Agilent's recommendations.
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| Contributor(s) |
Revert F, Macías J, Saus J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 16, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Fernando Revert |
| E-mail(s) |
fernando@cipf.es
|
| Organization name |
Centro de Investigación Príncipe Felipe
|
| Lab |
Revert
|
| Street address |
Autopista del Saler 16
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46012 |
| Country |
Spain |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA147391 |
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