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| Status |
Public on Nov 18, 2011 |
| Title |
A Systematic Substrate Screen Links Cyclin D-Dependent Kinases to Senescence Suppression in Cancer Cells through FOXM1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression (mRNA profiling) of U2OS osteosarcoma cells treated with 1 mM of the CDK4/6-specific inhibitor, PD0332991, versus vehicle (DMSO) for 4 hours
Here, we monitored the early transcriptional response to acute inhibition of CDK4/6 catalytic activity. As PD0332991 substantially induced senescence in U2OS cells, we performed gene expression arrays from U2OS cells treated with or without PD0332991 for 4 hours. Although this did not result in significant changes in the cell cycle profile, it led to significant changes in global gene expression.
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| Overall design |
10 total samples were analyzed, i.e. we compared five PD0332991-treated versus five vehicle-treated U2OS cell samples (gene level).
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| Contributor(s) |
Anders L, Sicinski P |
| Citation(s) |
22094256 |
| Submission date |
Sep 16, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Lars Anders |
| E-mail(s) |
lars.anders.hms@gmail.com
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| Organization name |
Dana-Farber Cancer Institute
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| Department |
Cancer Biology
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| Street address |
44 Binney Street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM797662 |
U2OS with DMSO, biological rep4 |
| GSM797663 |
U2OS with DMSO, biological rep5 |
| GSM797664 |
U2OS with PD0332991, biological rep1 |
| GSM797665 |
U2OS with PD0332991, biological rep2 |
| GSM797666 |
U2OS with PD0332991, biological rep3 |
| GSM797667 |
U2OS with PD0332991, biological rep4 |
| GSM797668 |
U2OS with PD0332991, biological rep5 |
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| Relations |
| BioProject |
PRJNA147393 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32182_RAW.tar |
236.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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