|
| Status |
Public on Sep 19, 2011 |
| Title |
Cell-type independent MYC target genes reveal a primordial signature involved in biomass accumulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
The functions of key oncogenic transcription factors independent of context have not been fully delineated despite our richer understanding of the genetic alterations in human cancers. The MYC oncogene, which produces the Myc transcription factor, is frequently altered in human cancer and is a major regulatory hub for many cancers. In this regard, we sought to unravel the primordial signature of Myc function by using high-throughput genomic approaches to identify the cell-type independent core Myc target gene signature. Using a model of human B lymphoma cells bearing inducible MYC, we identified a stringent set of direct Myc target genes via chromatin immunoprecipitation (ChIP), global nuclear run-on assay, and changes in mRNA levels. We also identified direct Myc targets in human embryonic stem cells (ESCs). We further document that a Myc core signature (MCS) set of target genes is shared in mouse and human ESCs as well as in four other human cancer cell types. Remarkably, the expression of the MCS correlates with MYC expression in a cell-type independent manner across 8,129 microarray samples, which include 312 cell and tissue types. Furthermore, the expression of the MCS is elevated in vivo in Em-Myc transgenic murine lymphoma cells as compared with premalignant or normal B lymphocytes. Expression of the MCS in human B cell lymphomas, acute leukemia, lung cancers or Ewing sarcomas has the highest correlation with MYC expression. Annotation of this gene signature reveals Myc's primordial function in RNA processing, ribosome biogenesis and biomass accumulation as its key roles in cancer and stem cells.
This SuperSeries is composed of the SubSeries listed below.
|
| |
|
| Overall design |
Refer to individual Series
|
| |
|
| Citation(s) |
22039435 |
| Submission date |
Sep 19, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Hongkai Ji |
| E-mail(s) |
hji@jhsph.edu
|
| Organization name |
Johns Hopkins Bloomberg School of Public Health
|
| Department |
Department of Biostatistics
|
| Street address |
615 North Wolfe Street, Rm E3638
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
| GPL5082 |
[Hs_PromPR] Affymetrix Human Promoter 1.0R Array |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (15)
|
|
| This SuperSeries is composed of the following SubSeries: |
| GSE31964 |
MYC binding in human B cells (P493-6) and ESC (H9) |
| GSE31965 |
Gene expression comparison between undifferentiated H9 cells and trophoblasts |
| GSE32219 |
Gene expression from human B-lymphocytes (P493-6) |
|
| Relations |
| BioProject |
PRJNA147219 |
Less...
More...