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Series GSE32360 Query DataSets for GSE32360
Status Public on Apr 03, 2013
Title Expression data from early Zebrafish embryos after knockdown of mir-34
Organism Danio rerio
Experiment type Expression profiling by array
Summary microRNAs play crucial roles in the early development of an organism. However the regulation of transcription through the action of microRNAs during the initial embyonic development has not been studied.
We used microarrays to detail the effect of maternal microRNA mir-34 in regulation of zygotic trancription during the initial stages of Zebrafish embryonic development from one cell stage through the maternal-zygotic transition up to 24 hours post fertilization.
 
Overall design Zebrafish embryos were selected at specific stages of early embryonic development (1, 7, and 24 hours post fertilization). RNA was extracted and hybridized to Affymetrix microarrays. The embryos were injected with anti-microRNA LNA and were kept for constant monitoring for the indicated time points. A mockLNA was injected as a control. The embryos were visualized for any visible developmental abnormalities.
 
Contributor(s) Pillai B, Soni K
Citation(s) 23470996
Submission date Sep 23, 2011
Last update date Jan 25, 2018
Contact name Kartik Soni
E-mail(s) kartik@igib.in
Organization name INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY
Department FGU
Lab MOL. BIO
Street address Mathura Road
City NEW DELHI
State/province NA
ZIP/Postal code 110028
Country India
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (18)
GSM801236 embryo injected with mockLNA, kept for 1hpf, biological rep1
GSM801237 embryo injected with mockLNA, kept for 1hpf, biological rep2
GSM801238 embryo injected with mockLNA, kept for 1hpf, biological rep3
Relations
BioProject PRJNA147985

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32360_RAW.tar 30.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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