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| Status |
Public on Apr 03, 2013 |
| Title |
Expression data from early Zebrafish embryos after knockdown of mir-34 |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
microRNAs play crucial roles in the early development of an organism. However the regulation of transcription through the action of microRNAs during the initial embyonic development has not been studied.
We used microarrays to detail the effect of maternal microRNA mir-34 in regulation of zygotic trancription during the initial stages of Zebrafish embryonic development from one cell stage through the maternal-zygotic transition up to 24 hours post fertilization.
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| Overall design |
Zebrafish embryos were selected at specific stages of early embryonic development (1, 7, and 24 hours post fertilization). RNA was extracted and hybridized to Affymetrix microarrays. The embryos were injected with anti-microRNA LNA and were kept for constant monitoring for the indicated time points. A mockLNA was injected as a control. The embryos were visualized for any visible developmental abnormalities.
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| Contributor(s) |
Pillai B, Soni K |
| Citation(s) |
23470996 |
| Submission date |
Sep 23, 2011 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Kartik Soni |
| E-mail(s) |
kartik@igib.in
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| Organization name |
INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY
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| Department |
FGU
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| Lab |
MOL. BIO
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| Street address |
Mathura Road
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| City |
NEW DELHI |
| State/province |
NA |
| ZIP/Postal code |
110028 |
| Country |
India |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (18)
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| GSM801236 |
embryo injected with mockLNA, kept for 1hpf, biological rep1 |
| GSM801237 |
embryo injected with mockLNA, kept for 1hpf, biological rep2 |
| GSM801238 |
embryo injected with mockLNA, kept for 1hpf, biological rep3 |
| GSM801239 |
embryo injected with anti-mir-34LNA, kept for 1hpf, biological rep1 |
| GSM801240 |
embryo injected with anti-mir-34LNA, kept for 1hpf, biological rep2 |
| GSM801241 |
embryo injected with anti-mir-34LNA, kept for 1hpf, biological rep3 |
| GSM801242 |
embryo injected with mockLNA, kept for 7hpf, biological rep1 |
| GSM801243 |
embryo injected with mockLNA, kept for 7hpf, biological rep2 |
| GSM801244 |
embryo injected with mockLNA, kept for 7hpf, biological rep3 |
| GSM801245 |
embryo injected with anti-mir-34LNA, kept for 7hpf, biological rep1 |
| GSM801246 |
embryo injected with anti-mir-34LNA, kept for 7hpf, biological rep2 |
| GSM801247 |
embryo injected with anti-mir-34LNA, kept for 7hpf, biological rep3 |
| GSM801248 |
embryo injected with mockLNA, kept for 24hpf, biological rep1 |
| GSM801249 |
embryo injected with mockLNA, kept for 24hpf, biological rep2 |
| GSM801250 |
embryo injected with mockLNA, kept for 24hpf, biological rep3 |
| GSM801251 |
embryo injected with anti-mir-34LNA, kept for 24hpf, biological rep1 |
| GSM801252 |
embryo injected with anti-mir-34LNA, kept for 24hpf, biological rep2 |
| GSM801253 |
embryo injected with anti-mir-34LNA, kept for 24hpf, biological rep3 |
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| Relations |
| BioProject |
PRJNA147985 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32360_RAW.tar |
30.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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