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| Status |
Public on Sep 28, 2011 |
| Title |
Class IA PI3Kinase regulatory subunit p85α regulates mast cell growth and survival related genes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Stem cell factor (SCF) mediated KIT receptor activation plays a pivotal role in mast cell growth, maturation and survival. However, the signaling events downstream from KIT are poorly understood. Mast cells express multiple regulatory subunits of class 1A PI3Kinase (PI3K) including p85α, p85β, p50α, and p55α. While it is known that PI3K plays an essential role in mast cells; the precise mechanism by which these regulatory subunits impact specific mast cell functions including growth, survival and cycling are not known. We show that loss of p85α impairs the growth, survival and cycling of mast cell progenitors (MCp). To delineate the molecular mechanism (s) by which p85α regulates mast cell growth, survival and cycling, we performed microarray analyses to compare the gene expression profile of MCps derived from WT and p85α-deficient mice in response to SCF stimulation. We identified 151 unique genes exhibiting altered expression in p85α-deficient cells in response to SCF stimulation compared to WT cells. Functional categorization based on DAVID bioinformatics tool and Ingenuity Pathway Analysis (IPA) software relates the altered genes due to lack of p85α to transcription, cell cycle, cell survival, cell adhesion, cell differentiation, and signal transduction. Our results suggest that p85α is involved in mast cell development through regulation of expression of growth, survival and cell cycle related genes.
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| Overall design |
Two-condition experiment, wildtype vs. p85α-deficient mast cell progenitors stimulated with SCF. Biological replicates: 3 wildtype replicates, 3 p85α-deficient replicates.
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| Contributor(s) |
Krishnan S, Mali R, Kapur R |
| Citation missing |
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| Submission date |
Sep 27, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Raghuveer Mali |
| Organization name |
Indiana University School of Medicine
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| Department |
Pediatrics
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| Street address |
1044 W Walnut St., R4 169
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| City |
Indianapolis |
| State/province |
Indiana |
| ZIP/Postal code |
46202 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (3) |
| GSM802217 |
WT mast cell rep1 vs p85α-KO mast cell rep1 |
| GSM802218 |
WT mast cell rep2 vs p85α-KO mast cell rep2 |
| GSM802219 |
WT mast cell rep3 vs p85α-KO mast cell rep3 |
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| Relations |
| BioProject |
PRJNA146389 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32410_RAW.tar |
43.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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