|
| Status |
Public on Sep 07, 2005 |
| Title |
MyoD, Myf5, myogenin, or hrGFP expressed in 2C5/7 myoD-/-;myf5-/- fibroblast cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression changes induced by MyoD or Myf5 were examined in a double-knockout fibroblast cell line lacking endogenous functional myoD or myf5 genes. Use of this cell line precluded the possibility of auto- or cross-activation of endogenous myoD or myf5. Myogenin or hrGFP were expressed in parallel samples as controls. Following infection with retrovirus - expressing the relevant myogenic regulatory factor (MRF) from the viral LTR promoter and hrGFP through an IRES element in the same mRNA transcript - GFP+ cells were sorted by FACS and harvested for total RNA.
Keywords: other
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| |
|
| Overall design |
this experiment include 4 samples and 12 replicates
|
| Web link |
http://www.stembase.ca/?path=/browse/experiment&id=223
|
| |
|
| Citation(s) |
16275751 |
| Submission date |
Sep 02, 2005 |
| Last update date |
Feb 18, 2018 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA92789 |
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