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Status |
Public on Jan 30, 2012 |
Title |
Gene expression profiling of mouse mammary tumorspheres, mammospheres, and mammospheres induced to differentiate |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Tumorpsheres and mammospheres were used to propagate mouse breast tumor-initiating cells and their normal stem/progenitor counterparts, respectively. Mammospheres induced to differentiate were used to model the more differentiated cells of the mouse mammary gland. We used microarrays to find differentrially expressed genes between tumorspheres, mammospheres, and mammospheres induced to differentiate
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Overall design |
Primary cells were isolate from either mouse mammary glands (FVB/N) or mouse mammary tumors and placed in stem cell media (MMTV-Neu [N2O2]. Spheres were passaged every 7 days for 3-5 passages and harvested for RNA isolation
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Contributor(s) |
Hassell JA |
Citation missing |
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Submission date |
Sep 28, 2011 |
Last update date |
Jan 08, 2019 |
Contact name |
Robin Hallett |
E-mail(s) |
halletrm@mcmaster.ca
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Organization name |
McMaster University
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Department |
Biochemistry and Biomedical Sciences
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Lab |
Hassell
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Street address |
1200 Main St W
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City |
Hamilton |
State/province |
Ontario |
ZIP/Postal code |
L8S 4L8 |
Country |
Canada |
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Platforms (1) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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Samples (9)
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GSM803271 |
Mammospheres Affy#858A-R_UMS |
GSM803272 |
Mammospheres Affy#859A_--UMS |
GSM803273 |
Mammospheres Affy#860A_--UMS |
GSM803274 |
Mammospheres induced to differentiate in serum Affy#861A_--DMS |
GSM803275 |
Mammospheres induced to differentiate in serum Affy#862A_--DMS |
GSM803276 |
Mammospheres induced to differentiate in serum Affy#863A_--DMS |
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Relations |
BioProject |
PRJNA147875 |