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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 31, 2011 |
| Title |
Expression data from porcine embryonic stem cells |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
We have been able to derive EpiSC-like pESC lines from in vivo produced porcine blastocysts. Our cell lines showed AP activity, expressions of the genes Oct4, Sox2, Nanog, Rex1, TDGF1, bFGF, FGFR1, FGFR2, Nodal and Activin-A involved in pluripotency and signaling pathways and in vitro differentiation potential, displaying similarities to epiblast stem cells or hES cells.
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| Overall design |
Porcine blastocysts were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs) in pESC medium. Following 5-7 days of culture, we observed EpiSC-like primary colonies derived from day 7 in vivo-produced. These EpiSC-like pESC colonies were mechanically dissociated into several clumps using pulled glass pipettes 10-15 days after seeding. Dissociated clumps were then re-seeded on fresh MEFs, and subsequent EpiSC-like pESC lines were routinely passaged via the pulled glass pipette method every 5-7 days. Our cell lines maintained stemness and a stable morphology for more than 56 passages. The main purpose of the present study was to investigate gloval gene expression from porcine embryonic stem cells.
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| Contributor(s) |
Son H, Park J, Kim H, Lee C |
| Citation(s) |
23326334 |
| Submission date |
Sep 30, 2011 |
| Last update date |
Jun 21, 2013 |
| Contact name |
Jinkyu Park |
| E-mail(s) |
parkjinkyu@snu.ac.kr
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| Phone |
+82 2 880 4805
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| Fax |
+82 2 873 4805
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| Organization name |
Seoul National University
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| Department |
Agricultural Biotechnology
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| Lab |
Animal Reproduction and Transgenesis
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| Street address |
Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
151-921 |
| Country |
South Korea |
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| Platforms (1) |
| GPL3533 |
[Porcine] Affymetrix Porcine Genome Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA147863 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32506_RAW.tar |
4.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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