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| Status |
Public on Apr 16, 2012 |
| Title |
Toxicogenomic responses of C. elegans to gold nanoparticles |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
We used Au nanoparticles (Au-NPs) as a model for studying particle specific effects of manufactured nanomaterials (MNMs) by examining the toxicogenomic responses in a model soil organism, free living nematode Caenorhabditis elegans. Global genome expression for nematodes exposed to 4-nm citrate-coated Au-NPs at the LC10 (5.9 mg L-1) revealed significant differential expression of 797 genes. The levels of expression for five genes (apl-1, dyn-1, act-5, abu-11, and hsp-4) were confirmed independently with qRT-PCR. Seven common biological pathways associated with 38 of these genes were identified. Activation of 26 pqn/abu genes from noncanonical Unfolded Protein Response (UPR) pathway and up-regulation of molecular chaperones (hsp-16.1, hsp-70, hsp-3 and hsp-4) were observed and are likely indicative of endoplasmic reticulum stress. Inhibition of abu-11 with RNAi showed increase in mortality in Au-NP exposed nematodes suggesting possible involvement of abu-11 (a gene associated with specific to C. elegans UPR) in a protective mechanism against Au-NPs. Exposure to Au-NPs also caused activation of genes involved in apoptosis and necrosis and resulted ultimately in 10% mortality. These results demonstrate that Au-NPs are bioavailable and cause adverse effects to a model ecoreceptor which activate both general and specific biological pathways.
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| Overall design |
Age-synchronized L3 nematodes (about 2000 nematodes per replicate with 3 replicates per treatment) were exposed to LC10 (5.9 mgL-1 ) of 4-nm citrate-coated Au-NP using 6 cm petri-dishes with 4 ml of exposure solution per dish. The N2 nematodes at L3 stageused were used and they were exposed in 50% K-Medium (31.68 mM KCl, 51.37 mM NaCl) for 12h. At 12 h all solutions were replaced with 50% K-Medium and left for another 12 h. After 24 h RNA was extracted from each of the replicates .
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| Contributor(s) |
Tsyusko O, Unrine J, Spurgeon D, Starnes D, Tseng M, Joice G, Bertsch P |
| Citation(s) |
22372763 |
| Submission date |
Sep 30, 2011 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Olga Tsyusko |
| E-mail(s) |
olga.tsyusko@uky.edu
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| Phone |
859-257-1777
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| Organization name |
University of Kentucky
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| Department |
Plant and Soil Sciences
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| Street address |
1100 S. Limestone St
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| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40546 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA147847 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32521_RAW.tar |
12.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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