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Series GSE32541 Query DataSets for GSE32541
Status Public on Sep 10, 2016
Title Gene expression in a 3D skin tissue subjected to bystander alpha-particle irradiation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The radiation bystander effect has been documented with multiple endpoints in various models. While many of the factors regulating this response are known, the mechanisms are still not well understood, and even less is known about signaling of bystander responses in 3-dimensional (3D) tissues. We have used the EPI-200 3D epidermal tissue model to measure global gene expression changes in bystander tissue in order to gain insight into the molecular pathways involved. We identified 187 genes with significantly altered expression in the region between 250 μm and 1 mm from the site of irradiation four hours after exposure. Gene expression at 250-500 μm and 500-750 μm was similar to that within the first 250 μm, the region that also included the directly irradiated tissue. Gene expression, although still significantly altered, began to return toward control levels in the region between 750 μm and 1 mm, however, suggesting a spatial limit to the bystander signal. The 3D bystander genes were concentrated in gene ontology classifications associated with direct irradiation, such as regulation of cell cycle progression and cell death, as well as categories previously associated with bystander responses, such as extracellular signaling, inflammatory processes, and terminal differentiation.
 
Overall design A 25-micron wide strip across the center of a 3D skin tissue model was exposed to either 0 or 0.5 Gy alpha particles, and gene expression was analyzed in slices of tissue located at various distances away from the site of irradiation 16 hours after exposure. Three biological repeats were performed for each condition
 
Contributor(s) Amundson SA, Mezentsev A
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Submission date Oct 01, 2011
Last update date Jan 23, 2019
Contact name Sally Amundson
E-mail(s) saa2108@cumc.columbia.edu
Organization name Columbia University
Department Center for Radiological Research
Street address 630 W. 168th St
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (24)
GSM806622 Control Center Rep1
GSM806623 Control 250 Rep1
GSM806624 Control 500 Rep1
Relations
BioProject PRJNA147807

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32541_RAW.tar 157.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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