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| Status |
Public on Dec 08, 2011 |
| Title |
Gene expression comparison of wild-type and IL15ko thymic NKT cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Semi-invariant natural killer T (NKT) cells are thymus-derived innate lymphocytes that modulate microbial and tumour immunity as well as autoimmune diseases. These immunoregulatory properties of NKT cells are acquired during their development. Much has been learnt regarding the molecular and cellular cues that promote NKT cell development, yet how these cells are maintained in the thymus and the periphery and how they acquire functional competence are incompletely understood. We found that IL-15 induced several Bcl-2 family survival factors in thymic and splenic NKT cells in vitro. Yet, IL15-mediated thymic and peripheral NKT cell survival critically depended on Bcl-xL expression. Additionally, IL-15 regulated thymic developmental stage 2 (ST2) to ST3 lineage progression and terminal NKT cell differentiation. Global gene expression analyses and validation revealed that IL-15 regulated Tbx21 (T-bet) expression in thymic ST3 NKT cells. The loss of IL15-dependent T-bet expression resulted in poor expression of IFN-γ and several NK cell receptors in NKT cells. Taken together, our findings reveal a critical role for IL-15 in NKT cell survival, which is mediated by Bcl-xL, and effector differentiation, which is regulated by T-bet.
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| Overall design |
Gene expression was measured in NKT cells sorted from pooled thymi of wild-type (3 replicates) or IL-15 deficient (2 replicates) mice.
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| Contributor(s) |
Gordy LE, Flyak AI, Joyce S |
| Citation(s) |
22084435 |
| Submission date |
Oct 03, 2011 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Joyce Sebastian |
| E-mail(s) |
sebastian.joyce@vanderbilt.edu
|
| Phone |
615-322-1472
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| Organization name |
Vanderbilt University School of Medicine
|
| Department |
Department of Microbiology and Immunology
|
| Street address |
A-4223 Medical Centre North
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA147009 |
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