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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 04, 2011 |
| Title |
Large-Scale Discovery of Enhancers from Human Heart Tissue |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Development and function of the human heart depend on the dynamic control of tissue-specific gene expression by distant-acting transcriptional enhancers. While large numbers of heart enhancers have been identified using the mouse as a model system, many of these regulatory sequences are poorly conserved in the human genome. To generate an accurate genome-wide map of human heart enhancers, we used an epigenomic enhancer discovery approach and identified ~6,200 candidate enhancer sequences directly from fetal and adult human heart tissue. Consistent with their predicted function, these elements were markedly enriched near genes implicated in heart development, function and disease. To further validate their in vivo enhancer activity, we tested 65 of these human sequences in a transgenic mouse enhancer assay and observed that 43 (66%) drove reproducible reporter gene expression in the heart. These results support the discovery of a genome-wide set of non-coding sequences highly enriched in human heart enhancers which is likely to facilitate down-stream studies of the role of enhancers in development and pathological conditions of the heart.
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| Overall design |
Examination of AcCBP/p300 binding in human adult heart, human fetal (16wk) heart and mouse postnatal day 2 heart
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| Contributor(s) |
Blow MJ, May D, Visel A |
| Citation(s) |
22138689 |
| Submission date |
Oct 04, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew James Blow |
| E-mail(s) |
mjblow@lbl.gov
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| Phone |
510-486-6590
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| Fax |
510-486-7004
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| Organization name |
Lawrence Berkeley National Laboratory
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| Department |
Genomics Division
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| Lab |
Rubin / Pennacchio
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| Street address |
1 Cyclotron Road
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (2) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (4)
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| Relations |
| SRA |
SRP008658 |
| BioProject |
PRJNA147085 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32587_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of BED, TXT, WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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