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| Status |
Public on Nov 01, 2011 |
| Title |
Distal airway stem cells yield alveoli in vitro and during lung regeneration following H1N1 influenza infection (colony) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The extent of lung regeneration following catastrophic damage and the potential role of adult stem cells in such a process remains obscure. Sublethal infection of mice with an H1N1 influenza virus related to that of the 1918 pandemic triggers massive airway damage followed by apparent regeneration. We show here that p63-expressing stem cells in the bronchiolar epithelium undergo rapid proliferation after infection and radiate to interbronchiolar regions of alveolar ablation. Once there, these cells assemble into discrete, Krt5+ pods and initiate expression of markers typical of alveoli. Gene expression profiles of these pods suggest that they are intermediates in the reconstitution of the alveolar-capillary network eradicated by viral infection. The dynamics of this p63-expressing stem cell in lung regeneration mirrors our parallel findings that defined pedigrees of human distal airway stem cells assemble alveoli-like structures in vitro and suggests new therapeutic avenues to acute and chronic airway disease.
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| Overall design |
3 colonies fron control and H1N1 influenza treated lungs (12 dpi) ,each were picked for whole genome microarray analysis
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| Contributor(s) |
Kumar PA, Hu Y, Yamamoto Y, Hoe NB, Wei TS, Mu D, Sun Y, Joo LS, Daghers R, Zielonka E, Wang DY, Chow VT, Crum CP, Xian W, McKeon F |
| Citation(s) |
22036562 |
| Submission date |
Oct 04, 2011 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Frank Daniel McKeon |
| E-mail(s) |
mckeonf@gis.a-star.edu.sg, Wa.Xian_imb.a-star.edu.sg
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| Organization name |
Genome Institute of Singapore
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| Department |
Stem cell and Developmental Biology 7
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| Street address |
60 Biopolis street
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE32606 |
Distal airway stem cells yield alveoli in vitro and during lung regeneration following H1N1 influenza infection |
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| Relations |
| BioProject |
PRJNA154451 |
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