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Series GSE32654 Query DataSets for GSE32654
Status Public on Jan 04, 2013
Title microRNA expression during ovine adult mammary gland development
Platform organism Mus musculus
Sample organisms Ovis aries; Mus musculus
Experiment type Non-coding RNA profiling by array
Summary The mammary gland undergoes extensive remodeling between the begin- ning of pregnancy and lactation; this involves cellular processes including cell proliferation, differentiation, and apoptosis, all of which are under the control of numerous regulators. To unravel the role played by miRNA, we describe here 47 new ovine miRNA cloned from mammary gland in early pregnancy displaying strong similari- ties with those already identified in the cow, human, or mouse. A microarray study of miRNA variations in the adult ovine mammary gland during pregnancy and lactation showed that 100 miRNA are regulated according to three principal patterns of expression: a de- crease in early pregnancy, a peak at midpregnancy, or an increase throughout late pregnancy and lactation. One miRNA displaying each pattern (miR-21, miR-205, and miR-200b) was analyzed by qRT- PCR. Variations in expression were confirmed for all three miRNA. Using in situ hybridization, we detected both miR-21 and miR-200 in luminal mammary epithelial cells when expressed, whereas miR-205 was expressed in basal cells during the first half of pregnancy and then in luminal cells during the second half. We therefore conclude that miR-21 is strongly expressed in the luminal cells of the normal mammary gland during early pregnancy when extensive cell prolif- eration occurs. In addition, we show that miR-205 and miR-200 are coexpressed in luminal cells, but only during the second half of pregnancy. These two miRNA may cooperate to maintain epithelial status by repressing an EMT-like program, to achieve and preserve the secretory phenotype of mammary epithelial cells.
 
Overall design 5 samples for sheep and 5 samples for mouse
 
Contributor(s) Galio L, Droineau S, Boudiaf H, Truchet S, Devinoy E
Citation(s) 23269700
Submission date Oct 05, 2011
Last update date Jan 30, 2018
Contact name Laurent GALIO
E-mail(s) laurent.galio@jouy.inra.fr
Organization name INRA
Lab Unité GPL
Street address Domaine de Vilvert
City JOUY-EN-JOSAS
ZIP/Postal code 78352
Country France
 
Platforms (1)
GPL8824 Agilent-019119 Mouse miRNA Microarray 1.0 G4472A (miRNA ID version)
Samples (10)
GSM810483 BREBIS G125
GSM810484 BREBIS G14
GSM810485 BREBIS G64
Relations
BioProject PRJNA147061

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32654_RAW.tar 16.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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