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| Status |
Public on Nov 01, 2011 |
| Title |
Genes affected upon dsRNA knockdown treatment for nbr/CG9247 in Drosophila DL1 cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
nbr/CG9247 gene function regulates the length of the 3'end of miRNAs.
Such 3'end heterogeneity of miRNAs may impact miRNA target gene silencing.
To address this, we performed microarray analysis for genes altered upon nbr knockdown in Drosophila DL1 cells.
This approach revealed a subset of genes upregulated upon nbr knockdown.
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| Overall design |
Five independent replicates of DL1 cells treated with control (dsRNA to renilla) or nbr (dsRNA RNA to nbr/CG9247) were collected 5 days after dsRNA bathing.
Total RNA from from each replicate was extracted using TRIzol reagent (Invitrogen).
A portion of total RNA for each replicate was used to confirm the effect of nbr knockdown on one of the nbr-dependent miRNAs, miR-34, by small RNA northern.
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| Contributor(s) |
Abe M, Bonini NM |
| Citation(s) |
22055292 |
| Submission date |
Oct 06, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Bonini Nancy |
| Organization name |
University of Pennsylvania
|
| Street address |
415 S University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (10)
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| GSM807162 |
Drosophila_DL1 cells_control RNAi 1 |
| GSM807163 |
Drosophila_DL1 cells_control RNAi 2 |
| GSM807164 |
Drosophila_DL1 cells_control RNAi 3 |
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| This SubSeries is part of SuperSeries: |
| GSE32564 |
Genes affected upon dsRNA knockdown treatment for nbr/CG9247 in Drosophila DL1 cells and small RNA profiling in Drosophila wild-type and nbr[f02257] mutants |
|
| Relations |
| BioProject |
PRJNA154375 |
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