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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 26, 2014 |
| Title |
Thrombospondin-1 type 1 repeats in a model of inflammatory bowel disease: genetic profile and therapeutic effects |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Thrombospondin 1 (TSP-1) is an anti-angiogenic matricellular protein with regulatory functions in inflammation and cancer. The type 1 repeats (TSR) domains of TSP-1 have been shown to interact with a wide range of proteins that result in the anti-angiogenic and anti-tumor properties of TSP-1. To evaluate potential therapeutic effects of TSRs in inflammatory bowel disease, we conducted clinical, histological and gene microarray analyses on a mouse model of induced colitis.
We used Affymetrix GeneChips to determine the changes in the genetic profile underlying TSR-treatment coincident with induction of colitis using DSS. We identified differentially expressed genes among the treatment groups.
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| Overall design |
Using DSS (dextran sulfate sodium) to induce colitis, wild-type mice were simultaneously injected with either saline or one form of TSP-1 derived peptides, containing either the three domains of the TSR (3TSR), the two last domains (TSR2), or TSR2 with the RFK sequence (TSR2+RFK). Wt mice drinking water only was used as reference.
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| Contributor(s) |
Gutierrez L, Lopez-Dee Z, Chittur SV |
| Citation(s) |
22509329 |
| Submission date |
Oct 07, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
schittur@albany.edu
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| Phone |
518-591-7215
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| Organization name |
SUNY-University at Albany
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| Department |
Center for Functional Genomics
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| Lab |
Microarray Core
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| Street address |
One Discovery Drive, CRC 342G
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| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (28)
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| GSM812400 |
Wt, DSS-3TSR treated, biological rep1 |
| GSM812401 |
Wt, DSS-3TSR treated, biological rep2 |
| GSM812402 |
Wt, DSS-3TSR treated, biological rep3 |
| GSM812403 |
Wt, DSS-3TSR treated, biological rep4 |
| GSM812404 |
Wt, DSS-TSR2 treated, biological rep1 |
| GSM812405 |
Wt, DSS-TSR2 treated, biological rep2 |
| GSM812406 |
Wt, DSS-TSR2 treated, biological rep3 |
| GSM812407 |
Wt, DSS-TSR2 treated, biological rep4 |
| GSM812408 |
Wt, DSS-TSR+RFK treated, biological rep1 |
| GSM812409 |
Wt, DSS-TSR+RFK treated, biological rep2 |
| GSM812410 |
Wt, DSS-TSR+RFK treated, biological rep3 |
| GSM812411 |
Wt, DSS-TSR+RFK treated, biological rep4 |
| GSM812412 |
Wt, DSS-saline treated, biological rep1 |
| GSM812413 |
Wt, DSS-saline treated, biological rep2 |
| GSM812414 |
Wt, DSS-saline treated, biological rep3 |
| GSM812415 |
Wt, DSS-saline treated, biological rep4 |
| GSM812416 |
Wt, water control, biological rep1 |
| GSM812417 |
Wt, water control, biological rep2 |
| GSM812418 |
Wt, water control, biological rep3 |
| GSM812419 |
Wt, DSS treated, biological rep1 |
| GSM812420 |
Wt, DSS treated, biological rep2 |
| GSM812421 |
Wt, DSS treated, biological rep3 |
| GSM812422 |
TSP-null-water, biological rep1 |
| GSM812423 |
TSP-null-water, biological rep2 |
| GSM812424 |
TSP-null-water, biological rep3 |
| GSM812425 |
DSS-treated TSP-null, biological rep1 |
| GSM812426 |
DSS-treated TSP-null, biological rep2 |
| GSM812427 |
DSS-treated TSP-null, biological rep3 |
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| Relations |
| BioProject |
PRJNA146891 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32697_RAW.tar |
116.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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