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| Status |
Public on Apr 10, 2012 |
| Title |
DNA methylation regulates lineage-specifying genes in the human vascular system [expression array]. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
During embryonic development, the lymphatic system emerges by transdifferentiation from the cardinal vein. Although lymphatic and blood vasculature share a close molecular and developmental relationship, they display distinct features and functions. However, even after terminal differentiation, transitions between the two endothelial cell types have been reported. Since changes in phenotypic plasticity and cellular differentiation processes frequently involve epigenetic mechanisms, we wondered whether DNA methylation might play a role in regulating cell type-specific expression in endothelial cells. By analyzing global gene expression and methylation patterns of primary human dermal lymphatic and blood endothelial cells, we identified a highly significant set of genes, which were differentially methylated and expressed. Pathway analyses of the differentially methylated and upregulated genes in lymphatic endothelial cells revealed involvement in developmental and transdifferentiation processes. We further identified a set of novel genes, which might be implicated in regulating BEC-LEC plasticity and could serve as therapeutic targets and/or biomarkers in vascular diseases associated with alterations in the endothelial phenotype.
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| Overall design |
Expression profile of 10 lymphatic endothelial cells was compared to that of 6 blood endothelial cells, no replicates, no control samples.
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| Contributor(s) |
Winnefeld M, Brönneke S, Hagemann S |
| Citation(s) |
22434260 |
| Submission date |
Oct 10, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Silvia Rueberg |
| Organization name |
Miltenyi Biotec GmbH
|
| Department |
Genomic Services Department
|
| Street address |
Friedrich-Ebert-Str. 68
|
| City |
Bergisch Gladbach |
| ZIP/Postal code |
51429 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE34487 |
DNA methylation regulates lineage-specifying genes in the human vascular system. |
|
| Relations |
| BioProject |
PRJNA155511 |
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