|
| Status |
Public on Oct 19, 2012 |
| Title |
MELK and UBE2C gene knockdown in prostate cancer cell line PC3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to address the putative role of MELK and UBE2C in prostate cancer development and progression, we performed functional analysis upon siRNA-based knockdown, and searched for downstream genes and processes by microarray experiments. RNAi-based inhibition of MELK and UBE2C was efficient in PC3 prostate cancer cells and decreased transcriptional level down to about 30% remaining expression level.
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| |
|
| Overall design |
Illumina microarray experiments were done upon siRNA based knockdown 48h after transfection of PC3 cells in triplicates.
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| |
|
| Contributor(s) |
Kuner R, Sültmann H, Fälth M, Chui-Pressinotti N, Klocker H |
| Citation(s) |
22945237 |
| Submission date |
Oct 11, 2011 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Ruprecht Kuner |
| Organization name |
German Cancer Research Center and National Center of Tumor Diseases
|
| Department |
Molecular Genetics
|
| Lab |
Unit Cancer Genome Research
|
| Street address |
Im Neuenheimer Feld 460
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (9)
|
| GSM813640 |
Human_Prostate_Cellline_PC3_knockdown_control_Allstar_1 |
| GSM813641 |
Human_Prostate_Cellline_PC3_knockdown_control_Allstar_2 |
| GSM813642 |
Human_Prostate_Cellline_PC3_knockdown_control_Allstar_3 |
| GSM813643 |
Human_Prostate_Cellline_PC3_knockdown_MELK_1 |
| GSM813644 |
Human_Prostate_Cellline_PC3_knockdown_MELK_2 |
| GSM813645 |
Human_Prostate_Cellline_PC3_knockdown_MELK_3 |
| GSM813646 |
Human_Prostate_Cellline_PC3_knockdown_UBE2C_1 |
| GSM813647 |
Human_Prostate_Cellline_PC3_knockdown_UBE2C_2 |
| GSM813648 |
Human_Prostate_Cellline_PC3_knockdown_UBE2C_3 |
|
| Relations |
| BioProject |
PRJNA146599 |
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