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Series GSE3296 Query DataSets for GSE3296
Status Public on Oct 24, 2005
Title Gene expression in MEFs in response to treatment with dipyridyl and trichostatin A
Organism Mus musculus
Experiment type Expression profiling by array
Summary The CH1 protein interaction domain of the transcriptional coactivators p300 and CBP is thought to interact with HIF-1alpha and this interaction is thought to be critical to the expression of HIF-1alpha target genes in response to hypoxia. Trichostatin A (TSA), an inhibitor of histone deacetylases, has been reported to repress the expression of HIF-1alpha target genes. To test the requirement of the CH1 domain and TSA for gene expression in response to dipyridyl (a hypoxia mimetic), primary mouse embryonic fibroblasts (MEFs) were generated from C57Bl/6x129/Sv F2 e14.5 embryos that contain a deletion in the CH1 domain of three of four alleles of CBP and p300. The remaining allele of p300 or CBP was a conditional knock out allele. Control MEFs with only a single conditional knockout allele of p300 or CBP were also generated. At passage 3 MEFs were infected with Cre Adenovirus and grown until they had expanded at least 100 fold. Subconfluent MEFs were treated with ethanol vehicle or 100ng/ml TSA with 5% carbon dioxide at 37 C in a humid chamber for 30 min., followed by ethanol vehicle or 100 umdipyridyl (DP) for an additional 3hrs. Immediately after treatment, cells were lysed in Trizol for RNA extraction.
Keywords: genetic modification, dose response
Overall design 16 samples; 4 genotypes [CBP+/flox (flox1), p300 +/flox (flox2), CBP CH1/flox;p300 CH1/CH1 (triCH1flox1),CBP CH1/CH1;p300 CH1/flox (triCH1flox2)] , 4 treatments (EtOH, TSA, dipyridyl (DP) and TSA+DP).
Contributor(s) Kasper LH, Boussouar F, Boyd K, Xu W, Biesen MA, Rehg J, Baudino TA, Cleveland JL, Brindle PK
Citation(s) 16237459
Submission date Sep 12, 2005
Last update date Feb 11, 2019
Contact name Paul K. Brindle
Phone 901 495 2451
Organization name St Jude Children's Research Hospital
Department Biochemistry
Street address 332 N Lauderdale
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM67338 flox1_EtOH
GSM67339 triCH1flox1_EtOH
GSM67340 flox2_2_EtOH
BioProject PRJNA92803

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