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Series GSE3303 Query DataSets for GSE3303
Status Public on Sep 16, 2005
Title Gene Expression Profiles of Intact and Regenerating Zebrafish Retina
Organism Danio rerio
Experiment type Expression profiling by array
Summary Purpose: Investigate the molecular determinants of retinal regeneration in adult vertebrates by analyzing the gene expression profiles of control and post-lesion retina of adult zebrafish, a system that regenerates following injury.
Methods: Gene expression profiles of zebrafish retina and brain were determined with DNA microarray, RT-PCR, and real-time quantitative PCR analyses. Damaged retinas and their corresponding controls were analyzed 2-5 days post-lesion (acute injury condition) or 14 d post-lesion (cell regeneration condition).
Results: Expected similarities and differences in the gene expression profile of zebrafish retina and brain were observed, confirming the applicability of the gene expression techniques. Mechanical lesion of retina triggered significant, time-dependent changes in retinal gene expression. The induced transcriptional changes were consistent with cellular phenomena known to occur, in a time-dependent manner, subsequent to retinal lesion, including cell cycle progression, axonal regeneration, and regenerative cytogenesis.
Conclusions: The results indicate that retinal regeneration in adult zebrafish involves a complex set of induced, targeted changes in gene transcription, and suggest that these molecular changes underlie the ability of the adult vertebrate retina to regenerate.
Keywords: time course; injury response; cellular correlation
Overall design Control brain and retina (unlesioned);
Control and lesioned retina (matched animals,
at least n = 8 for each condition).
Contributor(s) Cameron DA, Gentile KL, Middleton FA, Yurco P
Citation(s) 16205622
Submission date Sep 14, 2005
Last update date Jan 25, 2018
Contact name David A Cameron
Phone 315-464-8149
Fax 315-464-7712
Organization name SUNY Upstate
Department Neuroscience & Physiology
Street address 750 E Adams St
City Syracuse
State/province NY
ZIP/Postal code 13210
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (12)
GSM74260 Control Adult Zebrafish Brain
GSM74261 "true" control adult zebrafish retina
GSM74262 Control adult zebrafish retina, 2d post-lesion
BioProject PRJNA92815

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