GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE3313 Query DataSets for GSE3313
Status Public on Jun 30, 2014
Title Impaired revascularization in a mouse model of diabetes associated with dysregulation of angiogenic-regulatory network.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Diabetes is a risk factor for the development of cardiovascular diseases that are associated with impaired angiogenesis or increased endothelial cell apoptosis. Here is it shown that angiogenic repair of ischemic hind limbs was impaired in Lepr db/db mice, a leptin receptor deficient model of diabetes, compared to wild-type C57BL/6 (WT) mice as evaluated by laser Doppler flow and capillary density analyses. To identify molecular targets associated with this disease process, hind limb cDNA expression profiles were created from adductor muscle of Lepr db/db and WT mice before and after hind limb ischemia using Affymetrix GeneChip® Mouse Expression Set microarrays. The expression patterns of numerous angiogenesis related proteins were altered in Lepr db/db versus WT mice following ischemic injury. These transcripts included neuropilin-1, VEGF-A, placental growth factor, elastin and matrix metalloproteinases that are implicated in blood vessel growth and maintenance of vessel wall integrity. These data illustrate that impaired ischemia-induced neovascularization in type 2 diabetes is associated with the dysregulation of a complex angiogenesis-regulatory network.
Keywords: Diabetes, ischemia, angiogenesis, microarrays
Overall design Gene expression data for adductor muscle in the ischemic limb from wild type mice and Lepr db/db mice of 4 time points (before hind limb ischemia, 1 day, 7 days and 14 days after hind limb ischemia surgery). Three independent experimental replicates of each condition for each time point.
Contributor(s) Schiekofer S, Galasso G, Sato K, Kraus BJ, Walsh K
Citation(s) 15920034
Submission date Sep 15, 2005
Last update date Feb 11, 2019
Contact name Stephan Schiekofer
Organization name Boston University School of Medicine
Department Molecular Cardiology
Lab Kenneth Walsh Lab
Street address 700 Albany Street, W611
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (24)
GSM74466 WT1_hindlimb_d0_rep1
GSM74467 WT2_hindlimb_d0_rep2
GSM74468 WT3_hindlimb_d0_rep3
BioProject PRJNA93345

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
Raw data not provided for this record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap