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| Status |
Public on Oct 01, 2012 |
| Title |
Analysis of the early transcriptome signature of infection of primary endothelial cells by Nipah virus vs. Nipah virus deleted for the expression of non-structural C protein |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Nipah virus (NiV) is a highly pathogenic, negative strand RNA paramyxovirus that has recently emerged from flying foxes to cause serious human disease. To study the poorly-understood role of nonstructural NiV proteins in NiV pathogenesis, we generated recombinant viruse lacking the expression of accesory NiV C protein (NiV∆C).
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| Overall design |
To analyse the molecular basis of NiV∆C attenuation we have used the gene microarray approach to study early changes of gene expression in infected primary human endothelial cells, which is a major cellular target of human NiV infection.
We performed microarray gene expression profiling of NiV infected HUVEC cell (2 replicates), of uninfected HUVEC cell (2 replicates) and of NiV∆C infected HUVEC cell (2 replicates).
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| Contributor(s) |
MATHIEU C, GUILLAUME V, LEGRAS-LACHUER C, LACHUER J, HORVAT B |
| Citation(s) |
22837207, 22393386 |
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Mathieu Cyrille, Legras-Lachuer Catherine and Horvat Branka (2011). Transcriptome Signature of Nipah Virus Infected Endothelial Cells, Advances in the Etiology, Pathogenesis and Pathology of Vasculitis, Luis M. Amezcua-Guerra (Ed.), ISBN: 978-953-307-651-5, InTech; DOI: 10.5772/21512.
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| Submission date |
Oct 21, 2011 |
| Last update date |
Oct 28, 2014 |
| Contact name |
HORVAT Branka |
| E-mail(s) |
branka.horvat@inserm.fr
|
| Phone |
0437282392
|
| Fax |
0437282391
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| Organization name |
INSERM
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| Department |
human virology
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| Street address |
21 avenue Tony Garnier
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| City |
LYON |
| ZIP/Postal code |
69007 |
| Country |
France |
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| Platforms (1) |
| GPL2895 |
GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA149403 |
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