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Series GSE33174 Query DataSets for GSE33174
Status Public on Nov 15, 2011
Title Effects of acute dietary zinc depletion on the gene expression profile of whole blood in human male adults
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Even though the importance of adequate zinc intake has been known for around half a century, a reliable diagnostic tool to assess the dietary zinc status of individual humans or populations is in absence. The specific aim of this study was to examine differential expression of specific gene transcripts that occur when the dietary intake of zinc is acutely reduced below the dietary requirement for a period of ten days. Gene expression profiles of whole blood collected before and after dietary zinc restriction were determined by microarray analyses. The data provide potential signature genes of suboptimal zinc consumption and relevant bioinformatic interpretation indicate immune response and cell cycle regulation as biological processes associated with the zinc-responsive genes.
 
Overall design To identify candidate markers holding the potential to indicate zinc status, a 24-day observational study comprised of acclimation (7 d; 10.4 mg Zn/d), zinc depletion (10 d; 0.3 mg Zn/d), and zinc repletion (7 d; 29.5 mg Zn/d) phases was conducted with healthy male subjects (n = 9). On day 0, 6 and 10 of zinc depletion, whole blood was collectedunder morning fasting state. RNA profiles were stabilized by using PAXgene reagents during the collection process and globin RNA reduction was conducted to improve the detection of transcripts at low abundance. Genes responding to dietary zinc restriction were determined by array results from individual samples collected before and after 10 d of zinc depletion. Pooled RNA samples from each day of blood collection, i.e., baseline, 6 d and 10 d of depletion, respectively, were used to determine the temporal expression pattern of the zinc-responsive genes during dietary zinc depletion.
 
Contributor(s) Ryu M, Langkamp-Henken B, Chang S, Shankar MN, Cousins RJ
Citation(s) 22171008
Submission date Oct 24, 2011
Last update date Aug 13, 2018
Contact name Robert J. Cousins
E-mail(s) cousins@ufl.edu
Organization name University of Florida
Department Food Science and Human Nutrition Department, Center for Nutritional Sciences
Lab Nutritional Genomics Laboratory
Street address 359 FSHN Bldg. Newell Dr. PO Box 110370
City Gainesville
State/province Florida
ZIP/Postal code 32611-0370
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (24)
GSM821333 Baseline_PAX_pooled
GSM821334 ZnD-6d_PAX_pooled
GSM821335 ZnD-10d_PAX_pooled
Relations
BioProject PRJNA149223

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33174_RAW.tar 26.2 Mb (http)(custom) TAR
GSE33174_non-normalized.txt.gz 7.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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