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| Status |
Public on Dec 04, 2014 |
| Title |
Expression data from low- and high-pathogenicity avian influenza-infected chicken and duck cells |
| Platform organism |
Gallus gallus |
| Sample organisms |
Anas platyrhynchos; Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
While infection of chickens with highly pathogenic avian influenza (HPAI) H5N1 subtypes often leads to complete mortality within 24 to 48 h, infection of ducks in contrast causes mild or no clinical signs. Rapid onsets of fatal disease in chickens, but with no evidence of severe clinical symptoms in ducks, suggest underlying differences in their innate immune mechanisms. To understand the molecular basis for such difference, chicken and duck primary lung cells, infected with a low-pathogenicity avian influenza (LPAI) and two HPAI H5N1 viruses, were subjected to RNA expression profiling using Affymetrix Chicken GeneChip arrays.
We used microarrays to analyze the gene expression profiles of primary chicken and duck lung cells infected with H2N3 LPAI and two H5N1 influenza virus subtypes to understand the molecular basis of host susceptibility and resistance. We have identified a set of key genes and pathways that could play an important role in mediating innate host resistance to avian influenza in chickens and ducks.
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| Overall design |
24 hours following infection, total RNA from cells was extracted. Replicate RNA samples from each of the virus-infected (H2N3, H5N1 50-92, or H5N1 ty-Ty) or mock-infected chicken and duck cells (4 treatment groups for each species) were used for microarray analysis. Each of the RNA samples was hybridized to one GeneChip® Chicken Genome Array (Affymetrix), and a total of 16 array chips were used.
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| Contributor(s) |
Kuchipudi SV, Dunham SP, Chang K |
| Citation(s) |
25431115, 26484178, 32509599 |
| Submission date |
Nov 01, 2011 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Suresh Varma Kuchipudi |
| E-mail(s) |
suresh.kuchipudi@nottingham.ac.uk
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| Phone |
+44 (0) 115916653
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| Organization name |
University of Nottingham
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| Department |
School of Veterinary Medicine and Science
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| Lab |
Infection and Immunity
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| Street address |
College Road
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| City |
Sutton Bonington |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (16)
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| GSM825784 |
Chicken H2N3 infected, biological rep1 |
| GSM825785 |
Chicken H2N3 infected, biological rep2 |
| GSM825786 |
Chicken H5N1 50-92 infected, biological rep1 |
| GSM825787 |
Chicken H5N1 50-92 infected, biological rep2 |
| GSM825788 |
Chicken H5N1 ty-Ty infected, biological rep1 |
| GSM825789 |
Chicken H5N1 ty-Ty infected, biological rep2 |
| GSM825790 |
Chicken mock-infected control, biological rep1 |
| GSM825791 |
Chicken mock-infected control, biological rep2 |
| GSM825792 |
Duck H2N3 infected, biological rep1 |
| GSM825793 |
Duck H2N3 infected, biological rep2 |
| GSM825794 |
Duck H5N1 50-92 infected, biological rep1 |
| GSM825795 |
Duck H5N1 50-92 infected, biological rep2 |
| GSM825796 |
Duck H5N1 ty-Ty infected, biological rep1 |
| GSM825797 |
Duck H5N1 ty-Ty infected, biological rep2 |
| GSM825798 |
Duck mock-infected control, biological rep1 |
| GSM825799 |
Duck mock-infected control, biological rep2 |
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| Relations |
| BioProject |
PRJNA149119 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33389_RAW.tar |
48.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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