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Series GSE3339 Query DataSets for GSE3339
Status Public on Aug 08, 2006
Title Response of young and aged Mesenchymal Stem Cells to Dexamethasone
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary To explore the molecular events that underline skeletal physiology during aging we catalogued the profile of gene expression in ex vivo cultured MSCs derived from 3 and 15 month old rats and challenged with dexamethasone (Dex). RNA retrieved from these cells was analyzed using Affymetrix Gene Chips to compare the effect of Dex on gene expression.
Keywords: age effect, treatment
Overall design Mesenchymal Stem Cells were collected in the following manner: the bone marrow cells were flushed from the long bones, collected and prepared for single cells suspension. Cell pooled from six rats in each group were cultured in 75 cm2 flasks (Falcon, USA) containing 20 ml of growth medium, Dulbecco’s Modified Essential Medium (DMEM) containing 10% heat-inactivated fetal calf serum (FCS). Under these conditions, the hematopoietic cells died and the cultures finally remained only with cells forming the adherent stromal fibroblast-like layer. Cells were plated and after one week of culturing were trypsinized, single cell suspensions were re-cultured for 7 days and were grown in absence or presence of 10-8 M Dexamethasone (Dex). On day 14 cells were harvested for RNA extraction.

The groups included: young (3 month) untreated females, young (3 month) untreated males, young treated (Dex) females, old (15 month) untreated rats, old treated (Dex) rats.

Total RNAs were extracted from the four experimental groups using the TRIzol and cRNA prepared according to Affymetrix protocols. Gene expression was measured by hybridization to Affymetrix RAE230A Gene Chip DNA microarrays .
Contributor(s) Akavia UD, Shur I, Rechavi G, Benayahu D
Citation(s) 16643645
Submission date Sep 21, 2005
Last update date Mar 03, 2017
Contact name Dafna Benayahu
Organization name Tel Aviv University
Department Cellular and Developmental Biology, Sackler Faculty of Medicine
Street address POB 39040
City Tel Aviv
ZIP/Postal code 69978
Country Israel
Platforms (1)
GPL341 [RAE230A] Affymetrix Rat Expression 230A Array
Samples (5)
GSM75444 MSCs from young untreated male rats
GSM75445 MSCs from young (3months) untreated female rats
GSM75446 MSCs from old (15months) untreated female rats
BioProject PRJNA93247

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