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Series GSE33454 Query DataSets for GSE33454
Status Public on Feb 28, 2012
Title Matrix metalloproteinase-9 and TIMP-1 in peripheral nerve: Implications for myelin formation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Remyelination is a key step in functional nerve regeneration performed by Schwann cells (SC). We have demonstrated that matrix metalloproteinase (MMP)-9 is a major regulator of signal transduction and phenotypic switching in SCs. Herein, genome-wide transcriptional profiling, followed by Ingenuity Pathway Analysis revealed the MMP-9 signaling network and its endogenous inhibitor, TIMP-1, among the top induced genes of the injured sciatic nerve, that co-distributed with MMP-9 in myelinating SCs and the paranodal/nodal areas of myelinated fibers. Homo- and heterodimers of the active and proMMP-9 were purified from injured nerves using gelatin-sepharose. MMP-9 gene deletion increased the number of immature, GFAP+ mSC and post-mitotic cell counts that correlate with shorter myelin internodes in remyelinated fibers lacking MMP-9. MMP-9 is essential to nodal clustering of voltage-gated Na+ (Nav) channels. MMP inhibitor therapy diminished the expression of Nav 1.7 and 1.8. These data established the essential role of MMP-9 in guiding SC differentiation toward myelin production and in molecular assembly of the myelin domains. Modification of Nav channels in myelinated fibers may thus provide an important therapeutic approach for a number of facilitates regeneration and attenuated neuropathic pain.
 
Overall design Gene expression profiling of total RNAs extracted from murine sciatic nerves, dorsal root ganglion and spinal cords at day 1 and day 5 post injury.
 
Contributor(s) Chernov AV, Shubayev V, Strongin AY
Citation(s) 22438979
Submission date Nov 03, 2011
Last update date Jun 14, 2018
Contact name Andrei Chernov
E-mail(s) achernov@ucsd.edu
Phone 858 952 2854
Organization name University of California San Diego
Department Anesthesiology
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM827124 Normal_nerve_RN
GSM827125 D1_Injured_nerve_LND1
GSM827126 D5_Injured_nerve_LND1
Relations
BioProject PRJNA148677

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33454_RAW.tar 3.1 Mb (http)(custom) TAR
GSE33454_non-normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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