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Series GSE33519 Query DataSets for GSE33519
Status Public on Nov 10, 2011
Title Microarray profiling reveals broad and differential effects of RBM3 on miRNA expression.
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary MicroRNAs (miRNAs) are a family of short, noncoding RNAs that regulate translation of mRNAs by mechanisms involving the binding of complementary sequences. The influence of miRNAs on the proteome and cellular events is extensive as they regulate an estimated 60% of the transcriptome and play key roles in differentiation, plasticity, circadian rhythm, immunity, and disease. The post-transcriptional biogenesis of most miRNAs involves a sequential cleavage process mediated by RNase III family enzymes. Primary transcripts (pri-miRNAs) are first cleaved by Drosha in the nucleus to yield ~70nt hairpin precursors (pre-miRNAs). These intermediates are transported to the cytoplasm where ~22mer dsRNAs are excised by Dicer. Typically, one strand of the dsRNAs is inserted as a mature miRNA into the RNA-induced silencing complex (RISC), which contains members of the Argonaute (Ago) protein family that contribute to translational regulation. Recent studies indicate that some RNA-binding proteins (RNA-BPs) can regulate discrete processing steps, differentially blocking or promoting the formation of specific miRNAs to control cellular proliferation and differentiation. Elegant examples of this mechanism include the attenuation of let-7 biogenesis in embryonic stem cells by the pluripotency factor LIN28, and the selective enhancement of miR-18a biogenesis from a polycistronic transcript by hnRNPA1.
 
Overall design Mature miRNA expression profiles in B104 cells were measured after siRNA-mediated knockdown of RBM3 in the B104 neuronal cell line. Mock treated B104 cells were used as a control. Experiments were replicated in duplicates.
 
Contributor(s) Pilotte J, Dupont-Versteegden EE, Vanderklish PW
Citation(s) 22145045
Submission date Nov 07, 2011
Last update date Jan 30, 2018
Contact name Julie Pilotte
E-mail(s) pilotte.julie@gmail.com
Organization name The Scripps Research Institute
Department Neurobiology
Lab Peter Vanderklish
Street address 10550 North Torrey Pines Rd, Sbr-14
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL8824 Agilent-019119 Mouse miRNA Microarray 1.0 G4472A (miRNA ID version)
Samples (4)
GSM829374 B104 control rep1
GSM829375 B104 control rep2
GSM829376 B104 siRBM3 rep1
Relations
BioProject PRJNA148823

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33519_RAW.tar 2.5 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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