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| Status |
Public on Jun 04, 2013 |
| Title |
Distinct effects of topoisomerase II inhibitors on tumor cell lines (part 2) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected.
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| Overall design |
Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated control.
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| Contributor(s) |
Pang B, Velds A, Kerkhoven R, Neefjes J |
| Citation(s) |
23715267 |
| Submission date |
Nov 11, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Baoxu Pang |
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Michael Snyder
|
| Street address |
3165 Porter Drive
|
| City |
Palo Alto |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94304 |
| Country |
USA |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
|
| Samples (4)
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| GSM831673 |
MelJuSo cells 1 day post etoposide + aclarubicin |
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| This SubSeries is part of SuperSeries: |
| GSE33634 |
Topoisomerase II inhibitors and histone eviction |
|
| Relations |
| BioProject |
PRJNA154323 |
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