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| Status |
Public on Sep 22, 2006 |
| Title |
Regulation of hypothalamic gene expression by glucocorticoid: implications for energy homeostasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by SAGE
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| Summary |
The current study has investigated the hypothalamic gene expressions regulated by glucocorticoid (GC), key hormones in energy homeostasis. Using serial analysis of gene expression (SAGE) method, we have studied the effects of adrenalectomy (ADX) and GC on the transcriptomes of mouse hypothalamus. Approximately, 180 000 SAGE tags, which correspond to 50 000 tag species, were isolated from each group of intact or adrenalectomized mice, as well as 1, 3 and 24 hours after GC injection. ADX has upregulated diazepam binding inhibitor gene expression, while downregulating vomeronasal 1 receptor D4, genes involved in mitochondrial phosphorylation (cytochrome c oxidase 1 and NADH dehydrogenase 3), 3b-hydroxysteroid dehydrogenase-1, and prostaglandin D2 synthase. GC has increased the gene expression levels of dehydrogenase/reductase member 3, prostaglandin D2 synthase, solute carrier family 4 member 4, and five cytoskeletal proteins including myosin light chain phosphorylatable fast and troponin C2 fast. On the other hand, GC has reduced the mRNA levels of calmodilin 1 and expressed sequence tag similar to (EST) calmodilin 2, ATP synthase F0 subunit 6, and solute carrier family 4 member 3. Moreover, seven uncharacterized and 43 novel transcripts were modulated by ADX and GC. The current study has identified genes that may regulate hypothalamic systems governing energy balance in response to ADX and GC.
Keywords: Hormone effect analysis
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| Overall design |
Male C57BL6 mice were obtained from Charles River Laboratories (St. Constant, QC, Canada), at 12-14 weeks of age. Mice were housed in an air-conditioned room (19-25 degree) with controlled lighting from 07:15 to 19:15 h and were given free access to food (Lab Rodent Diet No. 5002) and water. One week prior to sampling of hypothalamus, adrenalectomy was performed in mice of all experimental groups (n = 12 per group). ADX mice received sodium chloride (0.9g/dl) in their drinking water after the surgery. GC (corticosterone, 0.1 mg per mouse) was subcutaneously injected to ADX mice and the hypothalamus was harvested at 1 hour (ADX + GC 1 h), 3 hours (ADX + GC 3 h) and 24 hours (ADX + GC 24 h) after the GC injection. ADX received an injection of vehicle solution (5% ethanol with 0.4% methocel A15LV premium) at 24 hours prior to sacrifice. All mice were killed between 08:30 and 12:30 by decapitation under isoflurane anesthesia. Brain was removed from the skull and the hypothalamus was immediately dissected, frozen in liquid nitrogen, pooled together for each group, and stored at -80 degree until RNA extraction.
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| Contributor(s) |
Nishida Y, Yoshioka M, St-Amand J |
| Citation(s) |
16368873 |
| Submission date |
Sep 26, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Yuichiro Nishida |
| E-mail(s) |
ynishida88@hotmail.com
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| Phone |
(717) 531-1028
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| Fax |
(717) 531-1792
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| Organization name |
Penn State College of Medicine
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| Department |
Department of Anatomy and Physiology
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| Lab |
Dr. Ray's Lab
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| Street address |
500 University Drive
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| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
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| Platforms (1) |
| GPL11 |
SAGE:10:NlaIII:Mus musculus |
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| Samples (5)
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| GSM38889 |
SAGE_hypothalamus_intact male mice |
| GSM75579 |
SAGE_hypothalamus_adrenalectomized male mice |
| GSM75581 |
SAGE_hypothalamus_adrenalectomized male mice 1h after glucocorticoid injection |
| GSM75582 |
SAGE_hypothalamus_adrenalectomized male mice 3h after glucocorticoid injection |
| GSM75584 |
SAGE_hypothalamus_adrenalectomized male mice 24h after glucocorticoid injection |
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| Relations |
| BioProject |
PRJNA93499 |
| Supplementary data files not provided |
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