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| Status |
Public on Dec 31, 2013 |
| Title |
Environmental pollution assessment using Mus spretus |
| Platform organism |
Mus musculus |
| Sample organism |
Mus spretus |
| Experiment type |
Expression profiling by array
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| Summary |
We evaluated the suitability of high-throughput transcriptomics approach to monitor terrestrial ecosystems. Free-living Mus spretus from several polluted sites of Huelva (Andalusia, SW Spain) were compared with mice from Doñana Biological Reserve (“Santa Olalla” lagoon (SOL) used as negative control). As most popular bioindicators, M. spretus is poorly represented in gene databases, therefore limiting the use of genomics in ecotoxicological studies. To solve this problem, we used microarrays produced from mRNAs of M. musculus. Specifically we used the One-Color Gene Expression Platform commercialized by Agilent.
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| Overall design |
Gene expression in Mus spretus liver was assessed in mice from 5 sites of the Spanish Southwest. Five independent experiments were performed. The arrays were hybridized with labelled cRNA samples made from pooled livers of 9 mice per sampling site. We made 4 technichal repetitions per sample.
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| Contributor(s) |
Ruiz-Laguna J, Abril N, Pueyo C |
| Citation(s) |
24460498 |
| Submission date |
Nov 14, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
Julia Ruiz-Laguna |
| E-mail(s) |
bb2rulaj@uco.es
|
| Organization name |
University of Córdoba
|
| Street address |
Campus Rabanales, Edif. Severo Ochoa 2ª pl.
|
| City |
Córdoba |
| ZIP/Postal code |
14071 |
| Country |
Spain |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA148635 |
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