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| Status |
Public on Aug 15, 2012 |
| Title |
Expression data from human memory CD4 T-cells stimulated with autologous monocytes pulsed with HCMV |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The present study reports an unbiased analysis of the genetic profile and regulation of NKG2D expressing CD4 T-cells.An Affymetrix microarray analysis was used to explore the genetic profile of NKG2D+ versus NKG2D- CD4 T-cells. The genetic profile was studied by single gene analysis and gene set enrichment analysis. I found that several immune regulatory receptors was regulated differently in NKG2D+ versus NKG2D- CD4 T-cells. Futhermore, I found that NKG2D+ CD4 T-cells display a genetic profile of cytotoxic T-cells. The gene set enrichment analysis revealed a change in 19 processes, including ARF GTPase activator activity; RNA splicing; Signal transduction; Interspecies interaction between organisms; Regulation of ARF GTPase activity; Cell motility; Mitosis; Cell cycle; Anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; Induction of apoptosis by extracellular signals; Negative regulation of apoptosis; mRNA export from nucleus; Positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; Cell division; Protein polymerization; Spliceosome assembly; Microtubule-based movement; Immune response; mRNA processing.
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| Overall design |
Human CD4 T-cells were isolated from buffy coat by anti-CD4 conjugated magnetic beads. Memory CD4 T-cells were sorted, labeled with CFSE and stimulated with autologous monocytes pulsed with HCMV. HCMV stimulated CD4 T-cells were sorted as CFSE-NKG2D+ or CFSE-NKG2D- and processed for microarray analysis.
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| Contributor(s) |
Jensen H |
| Citation(s) |
22870231 |
| Submission date |
Nov 14, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Helle Jensen |
| E-mail(s) |
hejen@life.ku.dk
|
| Organization name |
LIFE, University of Copenhagen
|
| Department |
Section of Biomedicine
|
| Lab |
Laboratory of Immunology
|
| Street address |
Stigbojlen 7
|
| City |
Frederiksberg C |
| ZIP/Postal code |
DK-1870 |
| Country |
Denmark |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
|
| GSM832602 |
NKG2D negative 1 (Biological replica 1) |
| GSM832603 |
NKG2D negative 2 (Biological replica 2) |
| GSM832604 |
NKG2D positive 1 (Biological replica 1) |
| GSM832605 |
NKG2D positive 2 (Biological replica 2) |
|
| Relations |
| BioProject |
PRJNA148731 |
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