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Series GSE33684 Query DataSets for GSE33684
Status Public on Jan 23, 2020
Title Fibroblast Growth Factor-21 Regulates PPARγ Activity and the Antidiabetic Actions of Thiazolidinediones
Organism Mus musculus
Experiment type Expression profiling by array
Summary Fibroblast growth factor-21 (FGF21) is a circulating hepatokine that beneficially affects carbohydrate and lipid metabolism. Here we report that FGF21 is also an inducible, fed-state autocrine factor in adipose tissue that functions in a feed-forward loop to regulate the activity of peroxisome proliferator-activated receptor γ (PPARγ), a master transcriptional regulator of adipogenesis. FGF21-knockout (KO) mice display defects in PPARγ signaling including decreased body fat and attenuation of PPARγ-dependent gene expression. Moreover, FGF21-KO mice are refratory to both the beneficial, insulin-sensitizing effects and the detrimental weight gain and edema side effects of the PPARγ agonist rosiglitazone. This loss of function in FGF21-KO mice is coincident with a marked increase in the sumoylation of PPARγ, which reduces its transcriptional activity. Adding back FGF21 prevents sumoylation and restores PPARγ activity. Collectively, these results reveal FGF21 as a key mediator of the physiologic and pharmacologic actions of PPARγ.
Overall design RNA was prepared from epididymal white adipose tissue of Diet-induced Obesity (DIO) wild-type and FGF21-KO mice treated with rosiglitazone or vehicle for 14 days (2 pools of 2 mice per condition).
Contributor(s) Yu RT, Dutchak PA, Kliewer SA
Citation(s) 22304921
Submission date Nov 14, 2011
Last update date Apr 23, 2020
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM832748 wild-type c57b6/sv129 mixed bkg WAT, DIO, biological replicate 1
GSM832749 wild-type c57b6/sv129 mixed bkg WAT, DIO, technical replicate of bio rep 1
GSM832750 wild-type c57b6/sv129 mixed bkg WAT, DIO, biological replicate 2
BioProject PRJNA148309

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33684_RAW.tar 3.1 Mb (http)(custom) TAR
GSE33684_non-normalized.txt.gz 4.7 Mb (ftp)(http) TXT
GSE33684_normalized.txt.gz 4.7 Mb (ftp)(http) TXT
GSE33684_statistics.txt.gz 139.6 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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