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Series GSE33690 Query DataSets for GSE33690
Status Public on Dec 15, 2011
Title MicroRNA profiles of four developmentally related hematopoietic cell types isolated from the BM of B6 and D2 mice.
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary performed a genetic screen for microRNAs that are differentially expressed between LSK, LS−K+, erythroid and myeloid cells isolated from C57BL/6 and DBA/2 mice. This analysis identified 131 microRNAs that were differentially expressed between cell types and 15 that were differentially expressed between mouse strains. Of special interest was an evolutionary conserved miR-cluster located on chromosome 17 consisting of miR-99b, let-7e and miR-125a. All cluster members were most highly expressed in LSKs and down-regulated upon differentiation. In addition, these microRNAs were higher expressed in DBA/2 cells compared to C57BL/6 cells, and thus correlated with HSPC frequency. To functionally characterize these microRNAs, we overexpressed the entire miR-cluster 99b/let-7e/125a and miR-125a alone in BM cells from C57BL/6 mice. Overexpression of the miR-cluster or miR-125a dramatically increased day-35 CAFC activity and caused severe hematopoietic phenotypes upon transplantation. We showed that a single member of the miR-cluster, namely miR-125a, is responsible for the majority of the observed miR-cluster overexpression effects. Finally, we performed genome-wide gene expression arrays and identified candidate target genes through which miR-125a may modulate HSPC fate.
 
Overall design Microarray-based microRNA profiling study was peformed of four developmentally related hematopoietic cell types isolated from the BM of B6 and D2 mice. Total RNA isolated from purified LSK multilineage cells, committed LS−K+ cells, erythroid TER-119+ cells and myeloid Gr-1+ cells was hybridized to Agilent microRNA arrays. Triplicates were generated for each of the 8 conditions (4 cell types, 2 mouse strains). From the final anaysis one replicate for B6 LS-K+ cells was removed based on PCA, therefore B6 Prog group consist of only 2 biological replicates.
 
Contributor(s) Gerrits A, Walasek MA, Olthof S, Weersing E, Ritsema M, Zwart E, van Os R, Bystrykh LV, de Haan G
Citation(s) 22123844
Submission date Nov 14, 2011
Last update date Jan 30, 2018
Contact name Leonid Bystrykh
E-mail(s) l.bystrykh@umcg.nl
Organization name University Medical Center Groningen
Department European Research Institute for the Biology of Ageing - ERIBA
Lab Ageing Biology and Stem Cells
Street address A. Deusinglaan 1
City Groningen
State/province Groningen
ZIP/Postal code 9713 AV
Country Netherlands
 
Platforms (1)
GPL8824 Agilent-019119 Mouse miRNA Microarray 1.0 G4472A (miRNA ID version)
Samples (23)
GSM832920 B6_Stem cells_(LSK)_rep1
GSM832921 B6_Stem cells_(LSK)_rep2
GSM832922 B6_Stem cells_(LSK)_rep3
This SubSeries is part of SuperSeries:
GSE33691 Genetic Screen Identifies MicroRNA Cluster 99b/let-7e/125a as a Regulator of Primitive Hematopoietic Cells
Relations
BioProject PRJNA154199

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33690_RAW.tar 37.9 Mb (http)(custom) TAR (of TXT)
GSE33690_processed_Cy3_miRNA.txt.gz 853.4 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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