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Status |
Public on Nov 16, 2011 |
Title |
The induction of integrin beta3 signaling in hematopoietic stem cells (HSCs) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
To determine the molecules involved in beta3 integrin signaling in HSCs, we carried out DNA microarray analyses using HSCs stimulated with ligand-mimic antibody for intetegrin beta3 in the presence of stem cell factor(SCF) and/or thrombopoietin (TPO). All experiments were performed in duplicate or triplicate using sorted cells that were independently prepared in separate experiments.
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Overall design |
After CD34-KSL HSCs were cultured with 2C9.G2 or control IgG for 5 days in S-Clone SF-03 medium supplemented with 50 ng/ml SCF and/or 50 ng/ml TPO, gene expression profiles were examimed using 5,000 sorted CD48-KSL cells from each sample.
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Contributor(s) |
Umemoto T, Yamato M, Eto K, Teruo O |
Citation(s) |
22096247 |
Submission date |
Nov 15, 2011 |
Last update date |
Jan 12, 2017 |
Contact name |
Terumasa Umemoto |
E-mail(s) |
tumemoto@abmes.twmu.ac.jp
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Organization name |
Tokyo women's medical university
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Street address |
8-1, Kawada-Cho
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City |
Shinjuku-Ku |
State/province |
Tokyo |
ZIP/Postal code |
162-8666 |
Country |
Japan |
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Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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Samples (16)
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Relations |
BioProject |
PRJNA148277 |