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| Status |
Public on Nov 16, 2011 |
| Title |
The induction of integrin beta3 signaling in hematopoietic stem cells (HSCs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To determine the molecules involved in beta3 integrin signaling in HSCs, we carried out DNA microarray analyses using HSCs stimulated with ligand-mimic antibody for intetegrin beta3 in the presence of stem cell factor(SCF) and/or thrombopoietin (TPO). All experiments were performed in duplicate or triplicate using sorted cells that were independently prepared in separate experiments.
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| Overall design |
After CD34-KSL HSCs were cultured with 2C9.G2 or control IgG for 5 days in S-Clone SF-03 medium supplemented with 50 ng/ml SCF and/or 50 ng/ml TPO, gene expression profiles were examimed using 5,000 sorted CD48-KSL cells from each sample.
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| Contributor(s) |
Umemoto T, Yamato M, Eto K, Teruo O |
| Citation(s) |
22096247 |
| Submission date |
Nov 15, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Terumasa Umemoto |
| E-mail(s) |
tumemoto@abmes.twmu.ac.jp
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| Organization name |
Tokyo women's medical university
|
| Street address |
8-1, Kawada-Cho
|
| City |
Shinjuku-Ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8666 |
| Country |
Japan |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA148277 |
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