|
| Status |
Public on Nov 15, 2011 |
| Title |
Protective Role for the Disulfide Isomerase PDIA3 in Methamphetamine Neurotoxicity |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by array
|
| Summary |
Methamphetamine abuse continues to be a worldwide problem, damaging the individual user as well as society. Only minimal information exists on molecular changes in the brain that result from methamphetamine administered in patterns typical of human abusers. In order to investigate such changes, we examined the effect of methamphetamine on the transcriptional profile in brains of monkeys. Gene expression profiling of the caudate and hippocampus identified protein disulfide isomerase family member A3 (PDIA3) to be significantly up-regulated in the animals treated with methamphetamine as compared to saline treated control monkeys. Treatment of primary rat neurons with methamphetamine revealed an up-regulation of PDIA3, showing a direct effect of methamphetamine on neurons to increase PDIA3. In vitro studies using a neuroblastoma cell line demonstrated that PDIA3 expression protects against methamphetamine-induced cell toxicity and methamphetamine-induced intracellular reactive oxygen species production, revealing a neuroprotective role for PDIA3. The current study implicates PDIA3 to be an important cellular neuroprotective mechanism against a toxic drug, and as a potential target for therapeutic investigations.
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| |
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| Overall design |
To study the effects of chronic METH effects on the brain
|
| |
|
| Contributor(s) |
Pendyala G, Fox HS |
| Citation(s) |
22715419 |
| Submission date |
Nov 15, 2011 |
| Last update date |
Aug 16, 2019 |
| Contact name |
Gurudutt Pendyala |
| E-mail(s) |
gpendyala@unmc.edu
|
| URL |
http://www.unmc.edu/pharmacology/fox.htm
|
| Organization name |
University of Nebraska Medical Center
|
| Department |
Pharmacology and Experimental Neuroscience
|
| Street address |
985800 Nebraska Medical Center
|
| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3535 |
[Rhesus] Affymetrix Rhesus Macaque Genome Array |
|
| Samples (12)
|
| GSM833165 |
Rhesus Siv+Meth,Hippo biological rep1 |
| GSM833166 |
Rhesus Siv+Meth,Hippo biological rep2 |
| GSM833167 |
Rhesus Siv+Meth,Hippo biological rep3 |
| GSM833168 |
Rhesus Ctrl,Caudate biological rep1 |
| GSM833169 |
Rhesus Ctrl,Caudate biological rep2 |
| GSM833170 |
Rhesus Ctrl,Caudate biological rep3 |
| GSM833171 |
Rhesus Siv+Meth,Caudate biological rep1 |
| GSM833172 |
Rhesus Siv+Meth,Caudate biological rep2 |
| GSM833173 |
Rhesus Siv+Meth,Caudate biological rep3 |
|
| Relations |
| BioProject |
PRJNA148485 |
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