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| Status |
Public on Nov 01, 2012 |
| Title |
Molecular events in endometrial carcinosarcomas and the role of high mobility group AT-hook 2 in endometrial carcinogenesis. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The molecular events implicated in the development of endometrial carcinosarcoma remain poorly understood. Using complementary DNA microarrays, we analyzed a group of 15 endometrial carcinosarcomas and compared their gene expression profiles with those obtained from a group of 23 endometrioid endometrial carcinomas. We demonstrated changes in the expression of genes modulating processes such as the epithelial to mesenchymal transition, muscle differentiation, the expression of cancer/testis antigens, and immune response in endometrial carcinosarcomas. The high mobility group AT-hook 2 gene is an embryonic nuclear factor that mediates epithelial to mesenchymal transition in various tumor models, and it was among the genes overexpressed in endometrial carcinosarcomas. High mobility group AT-hook 2 overexpression was confirmed in 54% of endometrial carcinosarcomas by quantitative real time-polymerase chain reaction and immunohistochemistry. Moreover, we found a significant inverse correlation between the expression of high mobility group AT-hook 2 and let-7b, a member of the let-7 family of microRNAs that represses high mobility group AT-hook 2 expression. These changes were also associated with overexpression of Lin28B, a suppressor of microRNA biogenesis that is implicated in cancer progression and metastasis. Finally, high mobility group AT-hook 2 overexpression, which was detected in less than 3% of endometrioid endometrial carcinomas, was observed in many nonendometrioid carcinomas (46% of 28 samples). This pattern of expression, restricted to nonendometrioid carcinomas and endometrial carcinosarcomas, reflects a role for high mobility group AT-hook 2 in endometrial carcinogenesis that is associated with aggressive phenotypes and points to its potential use as a marker to distinguish between endometrioid and nonendometrioid tumors.
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| Overall design |
15 endometrial carcinosarcomas and 23 endometrioid endometrial carcinoma
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| Contributor(s) |
Romero-Pérez L, Castilla MA, López-García MA, Díaz-Martín J, Biscuola M, Ramiro-Fuentes S, Oliva E, Matias-Guiu X, Prat J, Cano A, Moreno-Bueno G, Palacios J |
| Citation(s) |
22974476 |
| Submission date |
Nov 15, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Gema Moreno-Bueno |
| E-mail(s) |
gmoreno@iib.uam.es
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| Phone |
+34914978974
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| Organization name |
IIB
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| Department |
Biochemistry
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| Lab |
1.13
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| Street address |
Arturo Duperier
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
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| Platforms (2) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
| GPL6848 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Probe Name version) |
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| Samples (38)
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| Relations |
| BioProject |
PRJNA148455 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33723_RAW.tar |
588.5 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE33723_gene_level_processeed_data.txt.gz |
4.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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