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Series GSE33742 Query DataSets for GSE33742
Status Public on Apr 01, 2013
Title Drug-induced hepatotoxicity screening in primary mouse hepatocytes, an Omics approach.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Drug-induced hepatotoxicity is a leading cause of attrition of candidate drugs in drug development. Therefore new screening methods are necessary which predict these hazards more accurate and earlier in the drug development process. Of all in vitro hepatotoxicity models, primary human hepatocytes are considered as 'the gold standard'. However, the use of these hepatocytes is hindered by their scarcity and major inter-individual variation. These limitations may be overcome with use of primary mouse hepatocytes. Within this context changes in protein expressions in primary mouse hepatocytes, after exposure to cyclosporin A were studied using differential gel electrophoresis. Thereafter, the mRNA expression levels of these deregulated proteins from cyclosporin A-treated cells were analyzed. Cyclosporin A induced ER stress and altered the ER-Golgi transport, which may alter vesicle mediated transport and protein secretion. Moreover are the differentially expressed proteins observed upon challenge by cyclosporin A, associated with cholestatic mechanisms.
Overall design For each biological experiment, one hybridization was conducted and one sample per array. In total, 6 arrays were used for 2 different conditions (Csa or control at 48 hours).
Contributor(s) Van Summeren A, Renes J, Lizarraga D, Bouwman FG, Noben J, van Delft JH, Kleinjans JC, Mariman EC
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Submission date Nov 16, 2011
Last update date Apr 01, 2013
Contact name Anke Van Summeren
Organization name Maastricht University
Department Toxicogenomics
Street address Universiteitssingel 50
City Maastricht
ZIP/Postal code P.O. Box 616
Country Netherlands
Platforms (1)
GPL14661 [Mouse430_2_Mm_EntrezG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 13]
Samples (6)
GSM833946 AK9_PMH_48hr_0.5%_DMSO_1
GSM833947 AK25_PMH_48hr_0.5%_DMSO_2
GSM833948 AK41_PMH_48hr_0.5%_DMSO_3
BioProject PRJNA148421

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33742_RAW.tar 21.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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