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| Status |
Public on Feb 10, 2012 |
| Title |
Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a widespread and perhaps general feature of the gene expression program in human cells.
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| |
|
| Overall design |
3 samples of non-malignant primary human leukocytes, one replicate each
|
| |
|
| Contributor(s) |
Salzman J |
| Citation(s) |
22319583 |
| Submission date |
Nov 17, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Julia Salzman |
| E-mail(s) |
julia.salzman@gmail.com
|
| Organization name |
Stanford University
|
| Street address |
279 West Campus Drive, MC: 5307
|
| City |
Stanford |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (3) |
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| Relations |
| SRA |
SRP009373 |
| BioProject |
PRJNA148385 |
