|
| Status |
Public on Nov 23, 2011 |
| Title |
Eosinophil specific transcriptome in homeostatic intestine and lung |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Objective: To study the physiological role of eosinophils in the GI tract and lung under homeostatic conditions,
Method: we analyzed tissue-specific eosinophil gene expression patterns by genome-wide expression microarray analysis using RNA isolated from FACS-sorted eosinophils from the small intestine or lung of naive BALB/cmice (Live eosinophils were identified as DAPI-CCR3+Siglec-F+CD45+CD4-CD8a-CD19-B220-SSChigh cells).
Result: There are 513 genes that are differentially expressed by intestinal eosinophils and Lung eosinophils.
Conclusion: Eosinophils from different tissues have unique gene expression patterns for distinct functions.
|
| |
|
| Overall design |
we analyzed tissue-specific eosinophil gene expression patterns by genome-wide expression microarray analysis using RNA isolated from FACS-sorted eosinophils from the small intestine or lung of naive BALB/cmice (Live eosinophils were identified as DAPI-CCR3+Siglec-F+CD45+CD4-CD8a-CD19-B220-SSChigh cells).
|
| |
|
| Contributor(s) |
Rothenberg ME |
| Citation(s) |
22190185 |
| Submission date |
Nov 18, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Marc E Rothenberg |
| E-mail(s) |
rothenberg@cchmc.org
|
| Phone |
513-636-7177
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Allergy
|
| Lab |
Rothenberg
|
| Street address |
3333 Burnet Ave. MLC-7028
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA148127 |
Less...
More...