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| Status |
Public on Nov 01, 2005 |
| Title |
Differential susceptibility to Candida albicans in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
C5-deficiency in the A/J and recombinant congenic strain, BcA17, is linked to a dysregulated inflammatory response to Candida albicans infection and high morbidity compared to C5-sufficient C57Bl/6J (B6) mice. The recombinant congenic BcA70 strain thus provides an ideal model to study the effect of C5-deficiency on a B6 genetic background. We have used the A/J and BcA70 strains to carry out a detailed analysis of the pathophysiological effects of the uncontrolled inflammatory activity as a result of Candida infection. We present several lines of evidence to indicate that the heart of C5-deficient mice is severely affected by infection, although the kidney is the site of highest fungal replication. Histological sections of Candida-infected cardiac and kidney tissues from A/J mice reveal cellular infiltration in the vicinity of hyphal foci in the heart, leaving more dense fungal colonies in the kidney unaffected. As well as high-level inflammatory activity in the infected heart tissue, gene expression profiles revealed a striking lack of cardio-protective stress response linked to C5-deficiency. An inability of the A/J and BcA17 hearts to make the appropriate adaptive changes resulted in lower levels of both fatty acid and glucose metabolism, leading to cardiac failure.
Keywords: differential response to infection; genetic variation
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| Overall design |
4 biological replicates were used for each strain for both uninfected and infected samples and BcA70 was hybridized against control C57Bl/6 on chip. Dye swaps were included and normalization was performed using dye swap correction and intensity dependent normalization on GeneSpring.
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| Contributor(s) |
Mullick A, Leon Z, Min-Oo G, Berghout J, Lo R, Daniels E, Gros P |
| Citation(s) |
16861630, 21829669 |
| Submission date |
Sep 28, 2005 |
| Last update date |
Aug 19, 2019 |
| Contact name |
Gundula Min-Oo |
| E-mail(s) |
gundula.min-oo@mail.mcgill.ca
|
| Phone |
5143982542
|
| Organization name |
McGill University
|
| Department |
Biochemistry
|
| Street address |
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA93513 |
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