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Series GSE33863 Query DataSets for GSE33863
Status Public on Nov 22, 2011
Title mRNA-Seq reveals complex patterns of gene regulation and expression in the mouse skeletal muscle transcriptome associated with calorie restriction
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sarcopenia is an age-associated loss of skeletal muscle mass and strength that increases the risk of disability. Calorie restriction (CR), the consumption of fewer calories while maintaining adequate nutrition, mitigates sarcopenia and many other age-related diseases. To identify potential mechanisms by which CR preserves skeletal muscle integrity during aging, we used mRNA-Seq for deep characterization of gene regulation and mRNA abundance in skeletal muscle of old mice compared with old mice subjected to CR. mRNA-Seq revealed complex CR-associated changes in expression of mRNA isoforms, many of which occur without a change in total message abundance and thus would not be detected by methods other than mRNA-Seq. Functional annotation of differentially expressed genes reveals CR-associated upregulation of pathways involved in energy metabolism and lipid biosynthesis, and downregulation of pathways mediating protein breakdown and oxidative stress, consistent with earlier microarray-based studies. CR-associated changes not noted in previous studies involved downregulation of genes controlling actin cytoskeletal structures and muscle development. These CR-associated changes reflect generally healthier muscle, consistent with CR’s mitigation of sarcopenia. mRNA-Seq generates a rich picture of the changes in gene expression associated with CR, and may facilitate identification of genes that are primary mediators of CR’s effects.
 
Overall design Comprehensive survey of mRNA from skeletal muscle of mice subjected to calorie restricted or control diets using deep sequencing
 
Contributor(s) Dhahbi J
Citation(s) 22274562
Submission date Nov 22, 2011
Last update date Jul 20, 2018
Contact name joseph M dhahbi
E-mail(s) joseph.dhahbi@cnsu.edu
Phone 9166867891
Organization name California Northstate University College of Medicine
Department Medical Education
Lab Biochemistry
Street address 9700 West Taron Drive
City Elk Grove
State/province ca
ZIP/Postal code 95757
Country USA
 
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (2)
GSM838172 Control_muscle_RNA-Seq
GSM838173 CR_muscle_RNA-Seq
Relations
BioProject PRJNA148373

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33863_CR_Muscle_RNA-Seq_FPKM.txt.gz 573.4 Kb (ftp)(http) TXT
GSE33863_RAW.tar 125.3 Mb (http)(custom) TAR (of BEDGRAPH)
Raw data not provided for this record
Processed data are available on Series record
Processed data provided as supplementary file

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